THE APPLICATION OF PCR TO DIABETES RESEARCH

doi:10.13406/j.cnki.cyxb.1993.02.002

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THE APPLICATION OF PCR TO DIABETES RESEARCH

更新时间：2024-01-17

- THE APPLICATION OF PCR TO DIABETES RESEARCH
- Journal of Chongqing Medical University Issue 2, Pages: 101-105(1993)
- 作者机构：
  
  1. 重庆医科大学生物化学教研室
  2. 中国科学院上海生化所
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1993.02.002
  CLC：
- Published：1993，
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[1]刘先俊,吕懿娟,杜建国,冯佑民.PCR技术在糖尿病研究中的应用[J].重庆医科大学学报,1993(02):101-105+168.

THE APPLICATION OF PCR TO DIABETES RESEARCH. [J]. Journal of Chongqing Medical University (2):101-105(1993)
[1]刘先俊,吕懿娟,杜建国,冯佑民.PCR技术在糖尿病研究中的应用[J].重庆医科大学学报,1993(02):101-105+168. DOI： 10.13406/j.cnki.cyxb.1993.02.002.

THE APPLICATION OF PCR TO DIABETES RESEARCH. [J]. Journal of Chongqing Medical University (2):101-105(1993) DOI： 10.13406/j.cnki.cyxb.1993.02.002.

摘要

本文应用PCR技术从人基因组DNA中扩增出胰岛素基因片段（1128bp）

将扩增产物直接克隆到M

载体中去

用双脱氧链终止法作DNA序列分析。是鉴定胰岛素基因中前肽、A链、B链和C肽编码区域有无碱基突变的一种简便、有效的方法

Abstract

The polymerase chain reaction(PCR) was used to amplify insulin gene fragment (1128bp) in human genomic DNA. The amplified product was directly cloned to MI3 vector

and DNA sequencing was carried out by Sanger dideoxy-mediated chain termination method. This is a simple and effective method for identifying mutations in the coding region of prepeptide

A chain

B chain and C peptide of insulin gene

关键词

PCRDNA序列分析人胰岛素基因非胰岛素依赖型糖尿病

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