HYBRIDOMA AND MONOCLONAL ANTIBODY(McAb) FOR HEPATOCYTE STIMULATOR PROTEIN(HSP)

doi:10.13406/j.cnki.cyxb.1992.03.004

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HYBRIDOMA AND MONOCLONAL ANTIBODY(McAb) FOR HEPATOCYTE STIMULATOR PROTEIN(HSP)

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- HYBRIDOMA AND MONOCLONAL ANTIBODY(McAb) FOR HEPATOCYTE STIMULATOR PROTEIN(HSP)
- Journal of Chongqing Medical University Issue 3, Pages: 179-182(1992)
- 作者机构：
  
  1. 重庆医科大学病毒性肝炎研究所
  2. 重庆医科大学病毒性肝炎研究所研究生
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1992.03.004
  CLC：
- Published：1992，
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[1]周卫平,张定凤,刘帮媛,黄爱龙,刘林,贾小平.抗肝细胞生长多肽单克隆抗体及其抗独特型抗体的建立及初步应用[J].重庆医科大学学报,1992(03):179-182.

HYBRIDOMA AND MONOCLONAL ANTIBODY(McAb) FOR HEPATOCYTE STIMULATOR PROTEIN(HSP). [J]. Journal of Chongqing Medical University (3):179-182(1992)
[1]周卫平,张定凤,刘帮媛,黄爱龙,刘林,贾小平.抗肝细胞生长多肽单克隆抗体及其抗独特型抗体的建立及初步应用[J].重庆医科大学学报,1992(03):179-182. DOI： 10.13406/j.cnki.cyxb.1992.03.004.

HYBRIDOMA AND MONOCLONAL ANTIBODY(McAb) FOR HEPATOCYTE STIMULATOR PROTEIN(HSP). [J]. Journal of Chongqing Medical University (3):179-182(1992) DOI： 10.13406/j.cnki.cyxb.1992.03.004.

摘要

为了深入研究肝细胞生长因子及其与肝细胞作用的机制

本文应用单克隆抗体技术获得两株分泌肝细胞生长多肽单克隆抗体的杂交瘤细胞系:以纯化的两株单抗为抗原

我们制备了抗独特型兔血清抗体

发现它们具有肝细胞生长多肽样作用.本文应用单克隆抗体检测250例正常人和肝病病人血清（免疫斑点法）

发现重型肝炎病人血清肝细胞生长多肽含量明显增高

肝性脑病病人更为明显;慢性迁延性肝炎与正常人比较无显著差异.提示血清肝细胞生长多肽升高与肝细胞损伤程度有关.4例肝癌病人3例血清肝细胞生长多肽阳性

推测肝细胞生长多肽可能参与了肝细胞癌性生长的调控

Abstract

We have purified HSP from liver of human fetus by improved LaBrecque’ S method

Immunized BALB/C mice with HSP

we adopted activity-inhibition experiment of HSP as remedy for ELISA method to screen useful hybredoma clone

Finally

we obtained 2 antibodyproducing clonies

which produced lgG3 and IgGl that revealed clearly the dose-dependent inhibitive activity for HSP

The antiidiotype antibodies of anti-HSP showed similiar stimulative activity as HSP

Determining serum HSP by the anti-HSP

we discovered higher level .in patients with fulminant hepatic failure

关键词

单克隆抗体MeAb抗独特型抗体Anti-ld肝细胞生长多肽HSP

Keywords

Monoclonal Antibody McAbAnti-idiotype Antibody Anti-IdHepatocyte Stimulator Peptide HSP

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