HIGH-PERFORMANCE LIQUID CHROMATOGRATHY ASSAY FOR NIFEDIPINE IN BIOLOGICAL FLUIDS AND KINETICS OF NIFEDIPINE METABOLISM BY HUMAN LIVER MICROSOMES

doi:10.13406/j.cnki.cyxb.1992.03.001

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HIGH-PERFORMANCE LIQUID CHROMATOGRATHY ASSAY FOR NIFEDIPINE IN BIOLOGICAL FLUIDS AND KINETICS OF NIFEDIPINE METABOLISM BY HUMAN LIVER MICROSOMES

更新时间：2024-01-17

- HIGH-PERFORMANCE LIQUID CHROMATOGRATHY ASSAY FOR NIFEDIPINE IN BIOLOGICAL FLUIDS AND KINETICS OF NIFEDIPINE METABOLISM BY HUMAN LIVER MICROSOMES
- Journal of Chongqing Medical University Issue 3, Pages: 169-172(1992)
- 作者机构：
  
  1. 重庆医科大学药理教研室
  2. 重庆医科大学药理教研室中国医学科学院药物研究所,北京,100050
  3. 重庆医科大学药理教研室重庆医科大学药理教研室中国医学科学院药物研究所,北京,100050
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1992.03.001
  CLC：
- Published：1992，
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[1]何坎,全钰珠.硝苯吡啶的HPLC测定方法及在人肝微粒体内的代谢动力学[J].重庆医科大学学报,1992(03):169-172.

HIGH-PERFORMANCE LIQUID CHROMATOGRATHY ASSAY FOR NIFEDIPINE IN BIOLOGICAL FLUIDS AND KINETICS OF NIFEDIPINE METABOLISM BY HUMAN LIVER MICROSOMES. [J]. Journal of Chongqing Medical University (3):169-172(1992)
[1]何坎,全钰珠.硝苯吡啶的HPLC测定方法及在人肝微粒体内的代谢动力学[J].重庆医科大学学报,1992(03):169-172. DOI： 10.13406/j.cnki.cyxb.1992.03.001.

HIGH-PERFORMANCE LIQUID CHROMATOGRATHY ASSAY FOR NIFEDIPINE IN BIOLOGICAL FLUIDS AND KINETICS OF NIFEDIPINE METABOLISM BY HUMAN LIVER MICROSOMES. [J]. Journal of Chongqing Medical University (3):169-172(1992) DOI： 10.13406/j.cnki.cyxb.1992.03.001.

摘要

本文建立了测定生物样品中硝苯吡啶浓度的反相HPLC法.选用非那西丁作为内标物

固定相用Nova-Pak C18分析柱

流动相为甲醇-水（68:32）

紫外检测波长为240nm.样品由乙酸乙酯一次性提取

硝苯吡啶回收率为95.3±4.0%.本方法最低检测限为4ng.硝苯吡啶在人肝微粒体内代谢需NADPH再生系统

生成一个主要代谢物;按原药消除计算

硝苯吡啶代谢的Km和Vmax分别为58.0μmol/L和1.51nmol/mg/min

Abstract

A high-performance liquid chromatographic method was developed to determine nifedipine in biological fluids

by using ultraviolet detection at 240 nm.Phenacetin was chosen as internal standard.A Nova-Pak C18(4 um

3.9×150mm) was employed with methanol-water (68:32) as mobile phase. Nifedipine was extracted by ethyl acetate with a percentage of recovery about 95.3±1.0%. The limit of detection of this method for nifedipine was 4 ng for an injection volume of 20 ul. One major metabolite was formed from nifedipine after incubation with human liver microsom-es

the NADPH generating system was needed in this metabolic ptocess. Km and Vmax measured by nifedipine disappearance from roicrosomal incubation mixture were 58.0 umol/L and 1.51 nmol/mg/min

respectively

关键词

硝苯吡啶HPLC肝微粒体代谢

Keywords

NifedipineHPLCMetabolismMicrosomes

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