THE HIGH EXPRESSION OF HBV CORE ANTIGEN IN E.COLI

doi:10.13406/j.cnki.cyxb.1992.02.001

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THE HIGH EXPRESSION OF HBV CORE ANTIGEN IN E.COLI

更新时间：2024-01-17

- THE HIGH EXPRESSION OF HBV CORE ANTIGEN IN E.COLI
- Journal of Chongqing Medical University Issue 2, Pages: 81-85(1992)
- 作者机构：
  
  1. 重庆医科大学病毒性肝炎研究所
  2. 美国Johnson＆Johnson药理研究所副所长
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1992.02.001
  CLC：
- Published：1992，
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[1]黄爱龙,贾小平,张定凤,G.B.Thornton.乙型肝炎病毒核心抗原在大肠杆菌中的表达研究[J].重庆医科大学学报,1992(02):81-85.

THE HIGH EXPRESSION OF HBV CORE ANTIGEN IN E.COLI. [J]. Journal of Chongqing Medical University (2):81-85(1992)
[1]黄爱龙,贾小平,张定凤,G.B.Thornton.乙型肝炎病毒核心抗原在大肠杆菌中的表达研究[J].重庆医科大学学报,1992(02):81-85. DOI： 10.13406/j.cnki.cyxb.1992.02.001.

THE HIGH EXPRESSION OF HBV CORE ANTIGEN IN E.COLI. [J]. Journal of Chongqing Medical University (2):81-85(1992) DOI： 10.13406/j.cnki.cyxb.1992.02.001.

摘要

本文报道采用高效表达载体PBS（tac启动子）在大肠杆菌（E·coli）中表达乙型肝炎病毒核心抗原（HBcAg）的方法.重组质粒在异丙基一硫代半乳糖苷（IPTG）的诱导下

其表达效率提高近1000倍;经溶菌酶—SDS破壁、DNase处理后

离心上清液中含有大量HBcAg.通过对IPTG诱导浓度、诱导时间及细菌起始诱导浓度等因素的研究.我们找到一系列高效表达HB-cAg的条件

表达滴度高达1:25600

达到国内同类先进水平

Abstract

A method which uses high-level expression vector PBS (tae promoter) to express the HBV core antigen gone has been reported. Under the induction of iso-propylthio-β-D-galactosidc(IPTG)

the expression level of rccombinant plasmid h-as been increased 1000 times. After treatment with lysozyme-SDS-DNase system

the supernatant of crude bacterial lysale includes a large a mount of HBcAg.Through the studies of I1PTG induction concentration

IPTG induction time and the bacterial stale of initial induction

we have found a series of conditions to express the HBcAg gene efficiently. The highest liter of HBcAg is 1:25600

关键词

乙型肝炎核心抗原基因表达

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