THE PRODUCTION, CHARACTERIZATION AND PRELIMINARY APPLICATION OF TWO ANTI-VIMENTIN MCABS

Zhou Lan et al.

doi:10.13406/j.cnki.cyxb.1990.01.001

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THE PRODUCTION, CHARACTERIZATION AND PRELIMINARY APPLICATION OF TWO ANTI-VIMENTIN MCABS

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- THE PRODUCTION, CHARACTERIZATION AND PRELIMINARY APPLICATION OF TWO ANTI-VIMENTIN MCABS
- Journal of Chongqing Medical University Issue 1, Pages: 1-7(1990)
- 作者机构：
  
  重庆医科大学病理生理教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1990.01.001
  CLC：
- Published：1990，
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[1]周兰,范维珂,韦克,段积华.抗波形蛋白单克隆抗体的研制、鉴定及初步应用[J].重庆医科大学学报,1990(01):1-7+93-94.

Zhou Lan et al.. THE PRODUCTION, CHARACTERIZATION AND PRELIMINARY APPLICATION OF TWO ANTI-VIMENTIN MCABS. [J]. Journal of Chongqing Medical University (1):1-7(1990)
[1]周兰,范维珂,韦克,段积华.抗波形蛋白单克隆抗体的研制、鉴定及初步应用[J].重庆医科大学学报,1990(01):1-7+93-94. DOI： 10.13406/j.cnki.cyxb.1990.01.001.

Zhou Lan et al.. THE PRODUCTION, CHARACTERIZATION AND PRELIMINARY APPLICATION OF TWO ANTI-VIMENTIN MCABS. [J]. Journal of Chongqing Medical University (1):1-7(1990) DOI： 10.13406/j.cnki.cyxb.1990.01.001.

摘要

用从猪眼晶体中提取的波形蛋白免疫BALB/C小鼠;用杂交瘤技术建立了两株稳定分泌抗波形蛋白单克隆抗体（IgG

亚类）的杂交瘤细胞株:H

和I

;其染色体众数分别为和8690;免疫印迹显示两者均特异地与波形蛋白反应;免疫组化结果:纤维（母）细胞、巨噬细胞、浆细胞、内皮细胞、纤维瘤、恶纤、淋巴瘤、黑色素瘤等呈阳性反应;而神经原、肝、胰、肺鳞癌等呈阴性反应。此抗体的制成

对于正常组织和肿瘤组织的起源鉴定等有一定意义

Abstract

BALB/C mice were immunized with. Vimeatin. (Vim) isolated from pig lens. Two hybridoma cell liaes (H1C2 and I3B2) continuously secreting anti-Vim McAb (IgG1 subclass)were established by the hybridoma technique.Their chromosome modes are 86 and 90 respectively. The specificity of the two McAbs were tested with immu nob lotting and imrnunohistochemical technique on normal and tumor tissues. The results show that they reacted specifically with Vim in the blot and they are able to stain fibroblasts. fibrocytes. endothelial cells

fibroma

lymphoma. melanoma. etc; but not neuron

liver

pancreas

squamous cell carcinoma of lime

etc

Thein uses in the histoge.netic classification of normal and tumor tissues are briefly discussed

关键词

波形蛋白杂交瘤技术免疫印迹免疫组化肿瘤病理诊断

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