The metabolic characteristics of a transplantable hepatoma of inbred 615 mice(H615) was studied with 26 kinds of enzyme histcohemical methods. As compared with the normal mouse liver

it showed that the glycogen content was absent

the activity of ppr

SDH

ICDH

G-6-Pase

GPD

GDH and CCO was lowered

and the activity of HGK

PGI

LDH

G6PD

β-OH BD

NADPHD

NAE

γ-GT

ACP

β-GA

β-Gr

5’-N

and LAP was elevated. There were no changes in the enzyme activity of GPM

MDH

NADHD

ANAE

ATPase

and AKP. These results indicate that in the tumor tissue the synthesis of glycogen is decreased

the catabolism of glucose is enhanced with a preponderance in anaerobic glycolysis and a reduction in tricarboxylic acid cycle and oxidative phosphorylation. The gluconeegenems is also decreased

but the pentose pathway for nucleic acid synthesis is increased. It is accompanied by an increased transmembranous absorption of amino acids

hyperfuaction of intracellular lysozymes and the reappearance of a hepatocarcinoma marker γ-glutamyltranspeptidase. These changes are in accordance with the transformation of hepatic cells into hepatocarcinoma cells