DETERMINATION OF LEVOPRAZIQUANTEL IN SERUM BY A REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

doi:10.13406/j.cnki.cyxb.1988.01.023

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DETERMINATION OF LEVOPRAZIQUANTEL IN SERUM BY A REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

更新时间：2024-01-17

- DETERMINATION OF LEVOPRAZIQUANTEL IN SERUM BY A REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
- Journal of Chongqing Medical University Issue 1, Pages: 67-71(1988)
- 作者机构：
  
  1. 重庆医科大学血吸虫病研究室
  2. 重庆医科大学化学教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1988.01.023
  CLC：
- Published：1988，
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[1]钱明心,刘约翰,邱宗荫.测定左旋吡喹酮血药浓度的反相高效液相色谱法[J].重庆医科大学学报,1988(01):67-71.

DETERMINATION OF LEVOPRAZIQUANTEL IN SERUM BY A REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. [J]. Journal of Chongqing Medical University (1):67-71(1988)
[1]钱明心,刘约翰,邱宗荫.测定左旋吡喹酮血药浓度的反相高效液相色谱法[J].重庆医科大学学报,1988(01):67-71. DOI： 10.13406/j.cnki.cyxb.1988.01.023.

DETERMINATION OF LEVOPRAZIQUANTEL IN SERUM BY A REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. [J]. Journal of Chongqing Medical University (1):67-71(1988) DOI： 10.13406/j.cnki.cyxb.1988.01.023.

摘要

本文建立了测定左旋吡喹酮血药浓度的高效液相色谱分析方法.以ODS-C

化学键合相（5μm）作为固定相

甲醇:水（7:3V/V）作为流动相;用吡喹酮的衍生物作为内标准品.采用紫外检测器（λ=220nm）

得出检出限为2.5ng

最低检出血药浓度为0.012μg/ml血清

线性范围为0.012～1.6μg/ml血清（γ=0.999）

方法回收率为93.4～97.2%.本法简便、灵敏、专一、重现性好

适用于左旋吡喹酮血药浓度测定及药物动力学研究

Abstract

A HPLC method for quantitative determination of levopraziquantel in serum was described. A RP ODS-C18 (5μm) column fitted with a UV detector at 220 nm was used for analysis. The mobile phase used was methanol-water (7:3v/v). The analogue of praziquantel was used as an internal standard. The minimum detectable limit was 2.5ng. Assay linearity was demonstrated over the range of 0.012-1.6μg/ml of serum (r=0.999). The recovery ofthe method was 93.4-97.2%. This method was simple

precise

and sensitive

and had been used in the pharmacokinetic study and the clinical determination of levopraziquantel in serum.

关键词

左旋吡喹酮高效液相法血药浓度对映体

Keywords

1-praziquantelHPLCdrug conentration in serumenantiomer

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Related Author

钱明心

刘约翰

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Related Institution

重庆医科大学一院传染病教研室

重庆医科大学血吸虫病研究室重庆医科大学一院传染病教研室

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