THE EXTRACTION OF HUMAN KERATIN AND THE PREPARATION OF ANTIKERATIN ANTISERUM

doi:10.13406/j.cnki.cyxb.1987.04.001

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THE EXTRACTION OF HUMAN KERATIN AND THE PREPARATION OF ANTIKERATIN ANTISERUM

更新时间：2024-01-17

- THE EXTRACTION OF HUMAN KERATIN AND THE PREPARATION OF ANTIKERATIN ANTISERUM
- Journal of Chongqing Medical University Issue 4, Pages: 219-221(1987)
- 作者机构：
  
  1. 重庆医科大学理生理教研室
  2. 重庆医科大学病理生理教研室研究生
  3. 重庆医科大学理生理教研室重庆医科大学病理生理教研室研究生
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1987.04.001
  CLC：
- Published：1987，
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[1]苏绍文,范维珂.人角蛋白的提取与抗角蛋白抗血清的制备[J].重庆医科大学学报,1987(04):219-221+311-313.

THE EXTRACTION OF HUMAN KERATIN AND THE PREPARATION OF ANTIKERATIN ANTISERUM. [J]. Journal of Chongqing Medical University (4):219-221(1987)
[1]苏绍文,范维珂.人角蛋白的提取与抗角蛋白抗血清的制备[J].重庆医科大学学报,1987(04):219-221+311-313. DOI： 10.13406/j.cnki.cyxb.1987.04.001.

THE EXTRACTION OF HUMAN KERATIN AND THE PREPARATION OF ANTIKERATIN ANTISERUM. [J]. Journal of Chongqing Medical University (4):219-221(1987) DOI： 10.13406/j.cnki.cyxb.1987.04.001.

摘要

本研究用含8M尿素和0.1M巯基乙醇的Tris-HCl液从人胼胝中提取角蛋白。以十二烷基硫酸钠聚丙烯酰胺凝胶电泳测得其分子量为46

000、50

000、55

000和63

000。用电镜负染技术观察角蛋白细丝并测定其直径为80～100A。以此角蛋白免疫家兔制备成的抗血清

其琼扩效价为1∶32

用过氧化物酶—抗过氧化物酶法鉴定其特异性。该抗血清对正常上皮组织和上皮性肿瘤有反应

对非上皮性组织及其肿瘤无反应。初步证实:此抗血清具有抗角蛋白特异性

作为鉴定上皮性肿瘤的试剂具有一定价值

Abstract

Keratin was extracted from human callus with Tris-HCI buffer containing 8M urea and 0.1 M mercaptoethanol. The molecular weight was determined by SDS-PAGE as 46

000

000 and 63

000 daltons. Under negative staining electron microscopy

the keratin was a filament with a diameter of 80-100A. The antikerain antiserum was produced by immunizing keratin in rabbits. It showed a titre of Ii32 by agar diffusion. Its specificity was determined by peroxidase-antiperoxidase method. It reacts with normal epithelium and epithelial tumor tissues

but not with non-epthelial tissues and their tumors. So this antiserum is specific for keratin

and is useful as a reagent in the identification of epthelial tumors

关键词

角蛋白抗角蛋白抗血清上皮性肿瘤中间丝

Keywords

KeratinAntikeratin antiserumEpithelial tumorslatermediate filament

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