AN APPROACH TO THE METHODOLOGY IN ENZYMATIC DETERMINATION OF SERUM LECITHIN-CHOLESTEROL ACYLTRANSFERASE ACTIVITY

doi:10.13406/j.cnki.cyxb.1987.02.022

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AN APPROACH TO THE METHODOLOGY IN ENZYMATIC DETERMINATION OF SERUM LECITHIN-CHOLESTEROL ACYLTRANSFERASE ACTIVITY

更新时间：2024-01-17

- AN APPROACH TO THE METHODOLOGY IN ENZYMATIC DETERMINATION OF SERUM LECITHIN-CHOLESTEROL ACYLTRANSFERASE ACTIVITY
- Journal of Chongqing Medical University Issue 2, Pages: 122-124(1987)
- 作者机构：
  
  1. 重庆医科大学第二医院内科
  2. 重庆医科大学第二医院内科研究生
  3. 重庆医科大学第二医院内科重庆医科大学第二医院内科研究生
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1987.02.022
  CLC：
- Published：1987，
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[1]石小英,徐葆元.LCAT酶学测定法方法学探讨[J].重庆医科大学学报,1987(02):122-124.

AN APPROACH TO THE METHODOLOGY IN ENZYMATIC DETERMINATION OF SERUM LECITHIN-CHOLESTEROL ACYLTRANSFERASE ACTIVITY. [J]. Journal of Chongqing Medical University (2):122-124(1987)
[1]石小英,徐葆元.LCAT酶学测定法方法学探讨[J].重庆医科大学学报,1987(02):122-124. DOI： 10.13406/j.cnki.cyxb.1987.02.022.

AN APPROACH TO THE METHODOLOGY IN ENZYMATIC DETERMINATION OF SERUM LECITHIN-CHOLESTEROL ACYLTRANSFERASE ACTIVITY. [J]. Journal of Chongqing Medical University (2):122-124(1987) DOI： 10.13406/j.cnki.cyxb.1987.02.022.

摘要

<正>我们参照Nagasaki的卵磷脂胆固醇酰基转移酶（LCAT）活性测定法

以自身血清加入二软脂酰卵磷脂（DL）为底物

应用国产

酶试剂进行LCAT活性测定

并对方法学

特别是游离胆固醇显色反应进行了摸索。此方法具有两酶用量小

重复性好

操作简便的特点

适用于临床及实验室工作。

Abstract

A Nagasaki method with some modifications in determination of serum lecithin-cholesterol acyltransterase(LCAT)activity in 50 healthy people and 136 patienis with different kind of liver diseases is reported.The results suggested that the dttermination of serum LCAT activity might be taken as a sensitive parameter for monitoring impairment of liver function in patients with liver diseases. The methodology of enzymatic assay of serum LCAT activity

particularly in developing color of free cholesterol reaction is discussed

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