MICROENCAPSULATION OF ISLETS OF LANGERHANS Ⅱ . THE INSULIN SECRETION OF THE MICROENCAPSULATED RAT’S ISLETS DURING IN VITRO CULTURE USING CO2 AND COMMON INCUBATOR

doi:10.13406/j.cnki.cyxb.1987.02.006

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MICROENCAPSULATION OF ISLETS OF LANGERHANS Ⅱ . THE INSULIN SECRETION OF THE MICROENCAPSULATED RAT’S ISLETS DURING IN VITRO CULTURE USING CO2 AND COMMON INCUBATOR

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- MICROENCAPSULATION OF ISLETS OF LANGERHANS Ⅱ . THE INSULIN SECRETION OF THE MICROENCAPSULATED RAT’S ISLETS DURING IN VITRO CULTURE USING CO2 AND COMMON INCUBATOR
- Journal of Chongqing Medical University Issue 2, Pages: 89-92(1987)
- 作者机构：
  
  重庆医科大学第一医院人工细胞实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1987.02.006
  CLC：
- Published：1987，
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[1]舒昌达,何军,唐文淦.微囊包膜胰岛移植研究 Ⅱ 微囊包膜大鼠胰岛在CO_2及普通培养箱中培养时对比研究[J].重庆医科大学学报,1987(02):89-92.

MICROENCAPSULATION OF ISLETS OF LANGERHANS Ⅱ . THE INSULIN SECRETION OF THE MICROENCAPSULATED RAT’S ISLETS DURING IN VITRO CULTURE USING CO2 AND COMMON INCUBATOR. [J]. Journal of Chongqing Medical University (2):89-92(1987)
[1]舒昌达,何军,唐文淦.微囊包膜胰岛移植研究 Ⅱ 微囊包膜大鼠胰岛在CO_2及普通培养箱中培养时对比研究[J].重庆医科大学学报,1987(02):89-92. DOI： 10.13406/j.cnki.cyxb.1987.02.006.

MICROENCAPSULATION OF ISLETS OF LANGERHANS Ⅱ . THE INSULIN SECRETION OF THE MICROENCAPSULATED RAT’S ISLETS DURING IN VITRO CULTURE USING CO2 AND COMMON INCUBATOR. [J]. Journal of Chongqing Medical University (2):89-92(1987) DOI： 10.13406/j.cnki.cyxb.1987.02.006.

摘要

本文研究人工包膜包裹大鼠胰岛在CO

及普通培养箱中培养时胰岛分泌胰岛素情况

并认为包裹胰岛分泌胰岛素功能在培养液pH7.20～7.10左右为好。在研究中并发现即使用密封培养瓶在普通培养箱培养

不另加入CO

气体

亦能获得良好的培养效果

Abstract

In this paper

the secretion of the insulin of the microencapsulated new born rat’s islets during in vitro culture using CO2 and common incubators was studied. The secretion of insulin was more closely related to the pH of the culture media

and the most suitable pH was 7.1 to 7.2. It was also demonstrated that in stoppered flasks

placed in common incubator

the micro-encapsulatea islets were capable of producing sufficient metabolic CO2 to satisfy their nutrition requirement

关键词

细胞培养包膜胰岛胰岛移植

Keywords

cell cultureMicroencapsulated isletsTransplanation of islets of Langerhans

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