MODIFICATION OF WEISS METHOD IN THE DETERM INATION OF FACTOR VIII-RELATED RISTOCETIN CO-FACTOR

doi:10.13406/j.cnki.cyxb.1986.02.023

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MODIFICATION OF WEISS METHOD IN THE DETERM INATION OF FACTOR VIII-RELATED RISTOCETIN CO-FACTOR

更新时间：2024-01-17

- MODIFICATION OF WEISS METHOD IN THE DETERM INATION OF FACTOR VIII-RELATED RISTOCETIN CO-FACTOR
- Journal of Chongqing Medical University Issue 2, Pages: 126-128(1986)
- 作者机构：
  
  重庆医科大学第二医院内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1986.02.023
  CLC：
- Published：1986，
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[1]徐建业,刘祥贵,徐葆元.改良Weiss法测定因子Ⅷ相关瑞斯托毒素辅因子[J].重庆医科大学学报,1986(02):126-128.

MODIFICATION OF WEISS METHOD IN THE DETERM INATION OF FACTOR VIII-RELATED RISTOCETIN CO-FACTOR. [J]. Journal of Chongqing Medical University (2):126-128(1986)
[1]徐建业,刘祥贵,徐葆元.改良Weiss法测定因子Ⅷ相关瑞斯托毒素辅因子[J].重庆医科大学学报,1986(02):126-128. DOI： 10.13406/j.cnki.cyxb.1986.02.023.

MODIFICATION OF WEISS METHOD IN THE DETERM INATION OF FACTOR VIII-RELATED RISTOCETIN CO-FACTOR. [J]. Journal of Chongqing Medical University (2):126-128(1986) DOI： 10.13406/j.cnki.cyxb.1986.02.023.

摘要

<正> Ⅷ因子相关瑞斯托霉素辅因子（VIIIR:RCOF）的测定对凝血机理的探讨

以及假性血友病（VWD）的诊断与分型有较大意义。本文报告用改良的Weiss法

稍加改进

测定13例正常人与20例慢性肝病患者VIIIR:RCOF获得较为满意的结果。材料与方法试剂:3.8％枸橼酸钠1:9抗凝。Tris缓冲液（0.138M Nacl

0.05M KCl

0.015M Tris-HCl调至pH7.4）。Tris-EDTA缓冲液（1体积0.07MEDTA

49体积Tris缓冲液）。瑞斯托霉素（risto-cetin）系美国 BIO/DATA公司出品

牛血清白蛋白。

Abstract

Determination of factor VIII-related Co-factor (ristocetin) is of great importance to clarify the mechanism of blood coagulation and diagnosis and typing of pseudohemophilia (Von wil-kbrand’s disease) . A madified Weiss method in the determination of factor VIII-related ristocetin Co-factor in 13 healthy peoples and 20 patients with chronic liver disease is reported. The results of assay are satisfactory

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