Effect and mechanisms of heat shock pretreatment on bone marrow mesenchymal stem cells viability in chemotherapy environment

Li Xinran

doi:10.13406/j.cnki.cyxb.002802

您当前的位置：

首页 >

文章列表页 >

Effect and mechanisms of heat shock pretreatment on bone marrow mesenchymal stem cells viability in chemotherapy environment

更新时间：2024-01-09

- Effect and mechanisms of heat shock pretreatment on bone marrow mesenchymal stem cells viability in chemotherapy environment
- Journal of Chongqing Medical University Vol. 46, Issue 10, Pages: 1147-1152(2021)
- 作者机构：
  
  南方医科大学珠江医院妇产科,广州,510282
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.002802
  CLC： R711.75
- Published：2021，
扫描看全文
Li Xinran. Effect and mechanisms of heat shock pretreatment on bone marrow mesenchymal stem cells viability in chemotherapy environment. [J]. Journal of Chongqing Medical University 46(10):1147-1152(2021)
DOI：

Li Xinran. Effect and mechanisms of heat shock pretreatment on bone marrow mesenchymal stem cells viability in chemotherapy environment. [J]. Journal of Chongqing Medical University 46(10):1147-1152(2021) DOI： 10.13406/j.cnki.cyxb.002802.

摘要

目的：探索热休克预处理（heat shock pretreatment，HSP）对化疗微环境下骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs）的影响及其潜在机制。方法：探索HSP最佳条件，往热休克预处理BMSCs（heat shock pretreated bone marrow mesenchymal stem cells，HS-MSCs）培养基中加入顺铂，通过CCK-8检测细胞增殖率，Hoechst33342/PI测定存活率，流式细胞仪检测凋亡率。使用Western blot检测不同时间点HS-MSCs热休克蛋白70（heat shock protein 70，Hsp70）、Hsp90、自噬相关蛋白Beclin1和LC3B的表达。通过透射电镜观察自噬小体数量。结果：加入顺铂后，热休克模型组凋亡率明显低于模型组。此外，热休克模型组增殖速率较模型组增加，而表达亮蓝色/暗红色荧光的比例则低于模型组。HS-MSCs内Hsp70和Hsp90的表达随时间推移逐渐上调，并在48 h后达峰值。同时，细胞内自噬小体减少、Beclin1表达降低、LC3BⅡ/LC3BⅠ比值下降。结论： HSP能够提高化疗微环境下BMSCs的存活与抗凋亡能力，其作用可能与HSP增强Hsp70及Hsp90的表达、减弱自噬水平有关。

Abstract

Objective: This study aims to explore the potential effects and mechanisms of heat shock pretreatment (HSP) on bone marrow mesenchymal stem cells (BMSCs) in chemotherapy microenvironment. Methods: The optimal condition of HSP was identified. Cisplatin was added to the heat shock pretreated bone marrow mesenchymal stem cells (HS-MSCs) medium to simulate the chemotherapy microenvironment. The effect of HSP on the biological characteristics of HS-MSCs was explored. The proliferation of BMSCs was detected by CCK-8 assay

the viability was measured by Hoechst33342/PI

and the apoptosis was detected by flow cytometry. The potential mechanisms of HSP were further explored from the perspective of heat shock proteins and autophagy. The expression of heat shock protein 70 (Hsp70)

Hsp90

Beclin1 and LC3B after HSP were detected by Western blot. The number of autophagosomes was observed by transmission electron microscopy. Results: In simulated chemotherapy microenvironment

the apoptosis rate of heat shock model group was lower than that of model group. In addition

the proliferation rate of the heat shock model group was higher than that of the model group

while the ratio of bright blue/dark red fluorescence cells was lower than that of the model group. The expressions of Hsp70 and Hsp90 were significantly up-regulated with time and reached the peak at 48 hours after HSP. Meanwhile

the number of autophagosomes

the expression of Beclin1 and the ratio of LC3BⅡ/LC3BⅠwere all decreased after HSP.Conclusion: HSP can improve the viability and anti-apoptosis ability of BMSCs under the chemotherapy microenvironment

and its effect may be related to the enhancement of Hsp70 and Hsp90 expressions and the weakening of autophagy level.

关键词

热休克骨髓间充质干细胞化疗热休克蛋白自噬

Keywords

heat shockbone marrow mesenchymal stem cellschemotherapyheat shock proteinautophagy

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Picroside Ⅱ improves Aβ-induced learning and memory deficits in mice by enhancing autophagy

Comparison of the clinical efficacy of Disitamab Vedotin and postoperative chemotherapy in the treatment of HER-2 positive locally advanced urothelial carcinoma

Bakuchiol initiates autophagy to reduce β-phosphoglycerol-induced calcification of mouse vascular smooth muscle cells

3-methyladenine prevents sleep disturbance-induced liver injury by suppressing autophagy and endoplasmic reticulum stress

Effect of progressive muscle relaxation training on coping style and emotion of patients with lung cancer

Related Author

Li Zhaolun

Du Yehong

Wang Maoju

He Yan

Shi Xiuyu

Tian Qiuyun

Fan Yepeng

Ren Fengming

Related Institution

Pediatric Research Institute，National Clinical Research Center for Child Health and Disorders， Ministry of Education Key Laboratory of Child Development and Disorders，Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory Disorders， Children’s Hospital of Chongqing Medical University

Chongqing Institute of Medicinal Plant Cultivation， Chongqing College of Traditional Chinese Medicine

Department of Urology，The First Affiliated Hospital of Chongqing Medical University

Vascular Surgery Department，The Affiliated Hospital of Southwest Medical University；Institute of Cardiovascular Research，Key Laboratory of Medical Electrophysiology of Ministry of Education，Medical Electrophysiological Ley Laboratory of Sichuan Province

Department of Gastroenterology， Gulin People’s Hospital

⁰