Effect of aldehyde dehydrogenase 2 on monocyte-human aortic endothelial cells adhesion and its mechanisms

Liu Hong

doi:10.13406/j.cnki.cyxb.002720

您当前的位置：

首页 >

文章列表页 >

Effect of aldehyde dehydrogenase 2 on monocyte-human aortic endothelial cells adhesion and its mechanisms

更新时间：2024-01-09

- Effect of aldehyde dehydrogenase 2 on monocyte-human aortic endothelial cells adhesion and its mechanisms
- Journal of Chongqing Medical University Vol. 46, Issue 8, Pages: 927-932(2021)
- 作者机构：
  
  丽水市人民医院急诊医学科,丽水,323000
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.002720
  CLC： R197
- Published：2021，
扫描看全文
Liu Hong. Effect of aldehyde dehydrogenase 2 on monocyte-human aortic endothelial cells adhesion and its mechanisms. [J]. Journal of Chongqing Medical University 46(8):927-932(2021)
DOI：

Liu Hong. Effect of aldehyde dehydrogenase 2 on monocyte-human aortic endothelial cells adhesion and its mechanisms. [J]. Journal of Chongqing Medical University 46(8):927-932(2021) DOI： 10.13406/j.cnki.cyxb.002720.

摘要

目的：探讨乙醛脱氢酶2（aldehyde dehydrogenase 2，ALDH2）对单核细胞（human acute monocytic leukemia cells，THP-1）-人主动脉内皮细胞（human aortic endothelial cells，HAECs）黏附的影响及相关机制。方法：将HAECs分为对照（Control）组，ALDH2 NC组，ALDH2组，siRNA-ALDH2 NC组，siRNA-ALDH2-1、-2、-3组，Western blot及real-time PCR检测转染效果，细胞黏附实验检测THP-1-HAECs黏附能力，Western blot及real-time PCR检测核转录因子-κB（nuclear factor-kappa B，NF-κB）转录情况。结果：与Control组、ALDH2 NC组比较，ALDH2组中ALDH2 mRNA及蛋白表达量上调（P<0.05）；与Control组、siRNA-ALDH2 NC组比较，siRNA-ALDH2-1、-2、-3组中ALDH2 mRNA及蛋白表达量下调（P<0.05），其中siRNA-ALDH2-2组中ALDH2 mRNA及蛋白表达量最低。与Control组、ALDH2 NC组比较，ALDH2组中THP-1-HAECs黏附能力降低（P<0.05），细胞核中NF-κB表达量下调（P<0.05），细胞质中NF-κB表达量上调（P<0.05），红色荧光减弱（P<0.05）；与Control组、siRNA-ALDH2 NC组比较，siRNA-ALDH2组中THP-1-HAECs黏附能力提高（P<0.05），细胞核中NF-κB表达量上调（P<0.05），细胞质中NF-κB表达量下调（P<0.05），红色荧光增强（P<0.05）。结论： ALDH2通过抑制NF-κB核转录进而抑制THP-1-HAECs黏附，最终缓解动脉粥样硬化。

Abstract

Objective: To investigate the effect of aldehyde dehydrogenase 2 (ALDH2) on monocyte (THP-1) -human aortic endothelial cells (HAECs) adhesion and its mechanisms. Methods: HAECs was divided into the Control group

ALDH2 NC group

ALDH2 group

siRNA-ALDH2 NC group

siRNA-ALDH2-1

-2

-3 groups. The expression of ALDH2 protein and mRNA was detected by Western blot and real-time PCR. The adhesive ability between THP-1 and HAECs was detected by cell adhesion assay. The transcription of nuclear factor-kappa B (NF-κB) was detected by Western blot and immunofluorescence. Results: Compared with the Control group and the ALDH2 NC group

the expression of ALDH2 was up-regulated in the ALDH2 group (P<0.05). Compared with the Control group and the siRNA-ALDH2 NC group

the expression of ALDH2 was down-regulated in the siRNA-ALDH2-1

-2

-3 groups (P<0.05)

and the expression of ALDH2 was the lowest in the siRNA-ALDH2-2 group. Compared with Control group and ALDH2 NC group

cell adhesive ability

the expression of NF-κB in nucleus and the red fluorescence was decreased (P<0.05)

and the expression of NF-κB in cytoplasm increased in ALDH2 group (P<0.05). Compared with Control group and siRNA-ALDH2 NC group

cell adhesive ability

the expression of NF-κB in nucleus and the red fluorescence was increased (P<0.05)

the expression of NF-κB in cytoplasm decreased in siRNA-ALDH2 group (P<0.05). Conclusion: ALDH2 could inhibit the adhesion between THP-1-HAECs by inhibiting NF-κB nuclear transcription

and finally alleviate atherosclerosis.

关键词

乙醛脱氢酶2动脉粥样硬化核转录因子-κB细胞间黏附

Keywords

aldehyde dehydrogenase 2atherosclerosisnuclear factor-kappa Bintercellular cohesion

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of renal denervation on atherosclerosis in rabbits and its mechanism

Preliminary study on the diagnosis of carotid atherosclerosis by duplex ultrasonography

Study on the expression of PPARs and NFκB in endothciial cells of hyperlipidemic rats

Interventional effect of polygonum cuspidatum sieb.et Zucc.on NOS in atherosclerosis rabbits in vivo

Relationship among serum uric acid,C-reactive protein and the degree of carotid artery atherosclerosis in patients with essential hypertension

Related Author

Yin Yuehui

Mei Xia

Lu Jiayi

Tan Zhen

Yang Hanxuan

董为伟

谢文卫

周冀英

Related Institution

Department of Cardiology,Chongqing Zhongshan Hospital

Department of Cardiology,the Second Affiliated Hospital,Chongqing Medical University

重庆医科大学临床学院神经内科

重庆医科大学基础医学院组胚教研室

Department of Cardiology,College of Clinical Medicine,Chongqing Medical University

⁰