Expression identification and functional analysis of circFLNB in bone marrow-derived cells

Chen Xi; Zhao Xiaoqing; Zhang Ke

doi:10.13406/j.cnki.cyxb.002542

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Expression identification and functional analysis of circFLNB in bone marrow-derived cells

更新时间：2024-01-09

- Expression identification and functional analysis of circFLNB in bone marrow-derived cells
- Journal of Chongqing Medical University Vol. 46, Issue 5, Pages: 533-538(2021)
- 作者机构：
  
  中国医科大学生命科学学院发育细胞生物教研室,沈阳,110000
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.002542
  CLC： R329.2
- Published：2021，
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Chen Xi, Zhao Xiaoqing, Zhang Ke. Expression identification and functional analysis of circFLNB in bone marrow-derived cells. [J]. Journal of Chongqing Medical University 46(5):533-538(2021)
DOI：

Chen Xi, Zhao Xiaoqing, Zhang Ke. Expression identification and functional analysis of circFLNB in bone marrow-derived cells. [J]. Journal of Chongqing Medical University 46(5):533-538(2021) DOI： 10.13406/j.cnki.cyxb.002542.

摘要

目的：探讨环状RNA hsa_circ_0066336（circFLNB）在骨髓源性细胞中的表达，并分析其潜在功能。方法：选择骨髓源性细胞THP-1和Jurket作为模式细胞，通过设计背对背引物获得circRNA，分析circRNA在2种模式细胞中表达的差异。采用T-A克隆手段构建质粒，进行测序、比对。用Arraystar软件预测hsa_circ_0066336的靶标miRNA。从THP-1细胞中提取靶标miRNA后反转录，再以Real-time PCR检测。结果： ①在THP-1和Jurket细胞中成功扩增出circFLNB，结果显示其表达在2种模式细胞中没有统计学差异（P=0.105）。②克隆出含有hsa_circ_0066336的质粒，测序比对后找到了反向环状剪切位点。③通过Arraystar软件预测了hsa_circ_0066336靶向的5个miRNA，并检测到靶标miRNA的表达。结论： CircFLNB是表达在骨髓源性细胞中的一个环状RNA，通过对其下游靶向miRNA功能的了解，推断circFLNB可能会参与多种疾病的发生。

Abstract

Objective: To identify the expression of RNA hsa_circ_0066336 (circFLNB) in bone marrow-derived cells

and to analyze its potential function. Methods: Bone marrow-derived cells THP-1 and Jurket were selected as model cells. Divergent primers were designed

and differences of circRNA expression in two model cells were analyzed. T-A cloning was used to construct plasmids for comparing. Then Arraystar software was used to predict the target miRNA of hsa_circ_0066336. The target miRNA was extracted and reverse transcribed from THP-1

and then was detected by Real-time PCR. Results: ①CircFLNB was successfully amplified in THP-1 and Jurket cells

and statistical analysis showed that their expression was not significantly different in two model cells (P=0.105). ②The plasmid containing hsa_circ_0066336 was cloned

and a back-splice junction was found after comparing two sequences.③Five target miRNAs of hsa_circ_0066336 were predicted by Arraystar software and corresponding expressions were detected. Conclusion: CircFLNB is a circular RNA expressed in bone marrow derived cells and it is suggested that circFLNB may involve in the development of various diseases by comprehending its downstream miRNA function.

关键词

环状RNAhsa_circ_0066336骨髓源性细胞微小RNA

Keywords

circular RNAhsa_circ_0066336bone marrow derived cellmicroRNA

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