Effect of GRASP65 expression on proliferation,apoptosis,migration and invasion of gastric cancer cells

Yi Yongfen; Zhang Wanyu; Peng Doudou; Luo Yi

doi:10.13406/j.cnki.cyxb.001448

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Effect of GRASP65 expression on proliferation,apoptosis,migration and invasion of gastric cancer cells

更新时间：2024-01-17

- Effect of GRASP65 expression on proliferation,apoptosis,migration and invasion of gastric cancer cells
- Journal of Chongqing Medical University Vol. 42, Issue 12, Pages: 1614-1619(2017)
- 作者机构：
  
  1. 重庆医科大学基础医学院病理学教研室分子医学与肿瘤研究中心
  2. 重庆医科大学附属第一医院妇产科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001448
  CLC： R735.2
- Published：2017，
扫描看全文
Yi Yongfen, Zhang Wanyu, Peng Doudou, et al. Effect of GRASP65 expression on proliferation,apoptosis,migration and invasion of gastric cancer cells. [J]. Journal of Chongqing Medical University 42(12):1614-1619(2017)
DOI：

Yi Yongfen, Zhang Wanyu, Peng Doudou, et al. Effect of GRASP65 expression on proliferation,apoptosis,migration and invasion of gastric cancer cells. [J]. Journal of Chongqing Medical University 42(12):1614-1619(2017) DOI： 10.13406/j.cnki.cyxb.001448.

摘要

目的:探讨高尔基体堆叠蛋白65(Golgi reassembly stacking protein 65

GRASP65)在体外对人胃癌细胞增殖、凋亡、迁移和侵袭能力的影响。方法:首先

通过Western blot和RT-qRCR鉴别低分化胃癌细胞MKN-45、中分化胃癌细胞SGC-7901、高分化胃癌细胞MKN-28和正常胃黏膜细胞GES-1中GRASP65蛋白和mRNA的表达量。然后

用si RNA干扰技术通过Lipofectamine 2000瞬时转染下调和MKN-45细胞中GRASP65的表达量。用qRT-PCR和Western blot检测转染后各组细胞中GRASP65的蛋白和mRNA表达量以证实转染成功。最后

用CCK-8、流式细胞术、Transwell迁移和侵袭实验检测转染后的各组胃癌细胞增殖、凋亡、迁移和侵袭能力的改变。结果:Western blot和qRT-RCR结果显示MKN-45细胞和SGC-7901细胞中GRASP65的蛋白和mRNA表达量明显高于MKN-28细胞和GES-1细胞中GRASP65蛋白和mRNA表达量(P<0.05)

故选择低分化胃癌细胞MKN-45作为实验细胞株来下调GRASP65的表达量。qRT-PCR和Western blot结果证明转染成功

转染组GRASP65的mRNA和蛋白表达量与阴性对照组和空白对照组对比有统计学意义(P<0.05)。CCK-8、流式细胞术、Transwell迁移和侵袭实验均证明

下调MKN-45细胞的GRASP65的表达量可抑制胃癌细胞MKN-45体外增殖、迁移和侵袭能力

并促进细胞凋亡。结论:下调GRASP65的表达可明显减弱胃癌细胞的体外增殖能力

同时促进胃癌细胞的凋亡、体外侵袭和迁移。

Abstract

Objective:To investigate the effect of GRASP65 expression on proliferation

apoptosis

migration and invasion of gastric cancer cells in vitro.Methods:First

Western blot and qRT-PCR were carried out in three different differentiated gastric cancer cells（MKN-45

SGC-7901

MKN-28）and normal gastric mucosa cells（GES-1）to test the expression of GRASP65 protein and mRNA.Then

si RNA interference technique was used through Lipofectamine 2000 to transient transfection MKN-45 cells to knockdown GRAPS65 expression.Western blot and qRT-PCR were used to test GRAPS65 expression and confirm transfection success.Finally

CCK-8

flow cytometry

Transwell migration and invasion experiments were used to test the change of ability of cell proliferation

apoptosis

migration and invasion after transfection.Results:Western blot and qRT-PCR results showed that the expressions of GRASP65 protein and mRNA in MKN-45 and SGC-7901 were much higher than those in MKN-28 and GES-1.MKN-45 was chosen as experimental cells.Western blot and qRT-PCR results demonstrated that GRASP65 had been efficiently downregulated through si RNA tansfection.In addition

CCK-8

flow cytometry

Transwell chamber assays results showed that

GRASP65 knockdown can suppress growth

promote apoptosis

inhibit migration and invasion of gastric cancer cells in vitro.Conclusion:GRASP65 knockdown could significantly suppress growth

promote apoptosis

inhibit migration and invasion of gastric cancer cells MKN-45 in vitro.

关键词

GRASP65siRNA增殖凋亡迁移侵袭

Keywords

GRASP65siRNAproliferationapoptosismigrationinvasion

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