Activation of Wnt/β-catenin signaling pathway promotes anti-apoptotic genes bcl-2 and survivin expression in H9c2 cell subject to chronic hypoxia

Wang Haixia; Zhang Lei; Liu Lingjuan; Liu Xiaoyan

doi:10.13406/j.cnki.cyxb.001406

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Activation of Wnt/β-catenin signaling pathway promotes anti-apoptotic genes bcl-2 and survivin expression in H9c2 cell subject to chronic hypoxia

更新时间：2024-01-17

- Activation of Wnt/β-catenin signaling pathway promotes anti-apoptotic genes bcl-2 and survivin expression in H9c2 cell subject to chronic hypoxia
- Journal of Chongqing Medical University Vol. 42, Issue 11, Pages: 1509-1513(2017)
- 作者机构：
  
  1. 重庆医科大学附属儿童医院心血管内科
  2. 儿童发育重大疾病国家国际科技合作基地儿童发育疾病研究教育部重点实验室儿科学重庆市重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001406
  CLC： R725.4
- Published：2017，
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Wang Haixia, Zhang Lei, Liu Lingjuan, et al. Activation of Wnt/β-catenin signaling pathway promotes anti-apoptotic genes bcl-2 and survivin expression in H9c2 cell subject to chronic hypoxia. [J]. Journal of Chongqing Medical University 42(11):1509-1513(2017)
DOI：

Wang Haixia, Zhang Lei, Liu Lingjuan, et al. Activation of Wnt/β-catenin signaling pathway promotes anti-apoptotic genes bcl-2 and survivin expression in H9c2 cell subject to chronic hypoxia. [J]. Journal of Chongqing Medical University 42(11):1509-1513(2017) DOI： 10.13406/j.cnki.cyxb.001406.

摘要

目的:探讨Wnt/β-catenin信号转导通路在H9c2心肌细胞慢性缺氧适应中的作用及可能机制。方法:将37℃、5%O

、5%CO

、90%N

条件下培养的H9c2细胞作为实验组

低氧培养6、12、24、36、48、72 h时收集细胞;将37℃、21%O

、5%CO

条件下培养的细胞作为对照组。采用Western blot和real-time PCR技术检测β-catenin、抗凋亡因子bcl-2和survivin在不同培养时间点的表达变化。结果:5%O

培养的H9c2细胞与对照组相比

β-catenin蛋白在缺氧6、12、24、36 h时表达明显增加（P=0.000

P=0.018

P=0.001

P=0.000）

bcl-2及survivin表达较对照组明显增加（P=0.000

P=0.000）。结论:Wnt/β-catenin信号通路激活可能是H9c2心肌细胞慢性缺氧的适应机制之一

其部分可能通过上调抗凋亡因子bcl-2及survivin表达起作用。

Abstract

Objective:To explore the effect and mechanism of Wnt/β-catenin signaling pathway in H9 c2’s cell adaptation to chronic hypoxia. Methods:Embryonic rat-heart-derived H9 c2 cells were exposed to 37 ℃

5%O2

5%CO2

and 90%N2 as the experimental?group;cells were collected at 6

72 h. The cells were cultured under 37 ℃

21%O2

5%CO2 as control group. Expression of β-catenin

bcl-2 and survivin m RNA or protein at different time points were measured by using Western blot and real-time PCR.Results:Protein levels of β-catenin increased at 6

36 h compared with those of control group（P=0.000

P=0.018

P=0.001

P=0.000）. bcl-2 and survivin were significantly increased after β-catenin reaching high level of expression（P =0.000

P =0.000）.Conclusion:Activation of Wnt/β-catenin signaling pathway may contribute to H9 c2 cell adaptation’s to chronic hypoxia

partly through upregulating anti-apoptosis genes expression to promote cell survival.

关键词

H9c2细胞慢性缺氧Wnt/β-cateninBcl-2Survivin

Keywords

H9c2 cellchronic hypoxiaWnt/β-cateninBcl-2Survivin

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