DNA microarray screening for ovarian cancer associated fibroblasts

Hou Liangsi; Ye Xiaolin; Linghu Hua

doi:10.13406/j.cnki.cyxb.001401

您当前的位置：

首页 >

文章列表页 >

DNA microarray screening for ovarian cancer associated fibroblasts

更新时间：2024-01-17

- DNA microarray screening for ovarian cancer associated fibroblasts
- Journal of Chongqing Medical University Vol. 42, Issue 11, Pages: 1385-1389(2017)
- 作者机构：
  
  重庆医科大学附属第一医院妇产科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001401
  CLC： R737.31
- Published：2017，
扫描看全文
Hou Liangsi, Ye Xiaolin, Linghu Hua. DNA microarray screening for ovarian cancer associated fibroblasts. [J]. Journal of Chongqing Medical University 42(11):1385-1389(2017)
DOI：

Hou Liangsi, Ye Xiaolin, Linghu Hua. DNA microarray screening for ovarian cancer associated fibroblasts. [J]. Journal of Chongqing Medical University 42(11):1385-1389(2017) DOI： 10.13406/j.cnki.cyxb.001401.

摘要

目的:寻找上皮性卵巢癌(epithelial ovarian cancer

EOC)癌相关成纤维细胞(cancer associated fibroblasts

CAFs)和正常成纤维细胞(normal fibroblasts

NFs)中差异表达的m RNA并进行分析

为进一步阐明肿瘤间质中癌相关成纤维细胞影响卵巢癌细胞恶性行为的机制提供依据。方法:分别提取3例上皮性卵巢癌的CAFs及3例正常卵巢组织的NFs

抽提总RNA

进行全基因表达谱分析。对差异基因进行基因本体论(Gene Ontology

GO)富集及信号通路联合分析

推定相关差异基因生物学功能

并对MDM2、NR3C1等6个上调的差异基因进行荧光定量PCR验证。结果:对芯片结果的分析显示差异基因共1 636个。q RTPCR对MDM2、NR3C1等6个差异基因的验证结果与芯片结果相符

生物信息学分析显示上述差异基因与癌相关通路、肌动蛋白细胞骨架重塑通路等有关

提示可能参与了对卵巢癌细胞恶性行为的促进作用。结论:EOC肿瘤间质中的成纤维细胞存在调控癌细胞的差异基因

分析这些差异基因为进一步研究卵巢癌成纤维细胞调控癌细胞生物学行为的分子机制提供了基本依据。

Abstract

Objective:To seek the differential genes of cancer associated fibroblasts（CAFs）from normal fibroblasts（NFs）that might function in regulating the malignancy of cancer cells of EOC. Methods:CAFs and NFs were isolated from fresh ovarian tissue of patients of epithelial ovarian cancer（EOC）（n=3）and with benign diseases（n=3）. Total RNAs were extracted for detecting the differentially expressed genes between CAFs and NFs through DNA microarray. Gene Ontology and KEGG pathway analysis were conducted using DAVID to analyze the biological function of the deferentially expressed genes. q RT-PCR was applied to verify the reliability of DNA microarray result. Results:Totally 1 636 genes changed were identified in CAFs. q RT-PCR analysis of 6 genes validated the array results. Bioinformatics analysis indicated the likely involvement of these differential genes in carcinogenesis and regulation of actin cytoskeleton

which might promote the malignant behavior of EOC. Conclusion:Differentially expressed genes found in CAFs of EOC provide a basis for further studying the promotive role of CAFs for EOC.

关键词

上皮性卵巢癌癌相关成纤维细胞全基因表达谱芯片

Keywords

epithelial ovarian cancercancer associated fibroblastsGene expression profilingDNA microarray

references

Views

154

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

DNA microarray screening and analysis of the virulence gene in golden hamster cheek poch mucosa with precancerous lesions

Application of neoadjuvant chemotherapy and interval cytoreductive surgery in advanced epithelial ovarian cancer

Related Author

ZHANG Guo-dong

et al

梅杰

Yuan Hang

Zhang Shiqian

Related Institution

Department of Oral and Maxillofacial Surgery,the First Affiliated Hospital,Chongqing Medical University

Department of Obstetrics and Gynecology, Shandong Maternal and Child Health Hospital Affiliated to Shandong University

Department of Gynecology,Qilu Hospital of Shandong University

⁰