Effects of curcumin on the proliferation of medulloblastoma Daoy cells through down-regulating the expression of VEGF

Gao Minna; Zhang Xiong; Kang Qingmei; Liang Jie; Li Yu

doi:10.13406/j.cnki.cyxb.001373

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Effects of curcumin on the proliferation of medulloblastoma Daoy cells through down-regulating the expression of VEGF

更新时间：2024-01-17

- Effects of curcumin on the proliferation of medulloblastoma Daoy cells through down-regulating the expression of VEGF
- Journal of Chongqing Medical University Vol. 42, Issue 9, Pages: 1137-1142(2017)
- 作者机构：
  
  1. 重庆医科大学基础医学院病理教研室
  2. 重庆医科大学神经科学研究中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001373
  CLC： R739.4
- Published：2017，
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Gao Minna, Zhang Xiong, Kang Qingmei, et al. Effects of curcumin on the proliferation of medulloblastoma Daoy cells through down-regulating the expression of VEGF. [J]. Journal of Chongqing Medical University 42(9):1137-1142(2017)
DOI：

Gao Minna, Zhang Xiong, Kang Qingmei, et al. Effects of curcumin on the proliferation of medulloblastoma Daoy cells through down-regulating the expression of VEGF. [J]. Journal of Chongqing Medical University 42(9):1137-1142(2017) DOI： 10.13406/j.cnki.cyxb.001373.

摘要

目的:血管的新生与髓母细胞瘤的生长、侵袭和转移密切相关

本实验旨在观察姜黄素对髓母细胞瘤Daoy细胞的生长及其血管生长因子的影响

并探讨可能的机制。方法:体外培养髓母细胞瘤Daoy细胞

然后经不同浓度（5、10、20、40μmol/L）的姜黄素作用不同时间（24、48、72 h）后

通过MTT法检测姜黄素对Daoy细胞生长增殖的影响;ELISA法检测上清液中血管内皮生长因子（vascular endothelial growth factor

VEGF）的生成水平

Western blot检测Daoy细胞中Wnt/β-catenin通路中重要分子β-catenin以及VEGF蛋白水平的表达。结果:MTT结果示空白对照组、DMSO组、5μmol/L组、10μmol/L组、20μmol/L组和40μmol/L组作用24 h后

细胞的抑制率分别为（4.2±0.3）%、（4.3±0.5）%、（6.1±0.3）%、（6.9±0.7）%、（30.5±1.7）%和（40.9±2.4）%

差异具有统计学意义(F

浓度

=151.997

P=0.000);当20、40μmol/L的姜黄素作用48 h和72 h后

Daoy细胞的增殖抑制率分别为（41.3±2.0）%、（51.2±2.9）%、（48.4±1.6）%和（60.9±3.2）%

相较于作用24 h

差异也具有统计学意义(F

时间

=281.531

P=0.000;F

浓度×时间

=269.728

P=0.000)。ELISA结果显示:空白对照组、DMSO对照组、5μmol/L组、10μmol/L组、20μmol/L组、40μmol/L组细胞上清液中VEGF的含量分别为（237.5±2.5）、（248.4±3.0）、（235.6±7.1）、（230.8±3.8）、（198.2±9.9）、（150.5±3.6）pg/m L

差异具有统计学意义(F

浓度

=30.921

P=0.000)。当20μmol/L和40μmol/L的姜黄素作用48 h和72 h后

Daoy细胞上清液中生成的VEGF含量分别为（170.3±2.7）、（124.6±3.1）、（140.1±13.9）和（111.5±11.6）pg/m L

与作用24 h比较

差异也具有统计学意义(F

时间

=69.234

P=0.000;F

浓度×时间

=185.173

P=0.000)。Western blot结果显示

空白对照组、DMSO组、5μmol/L组、10μmol/L组、20μmol/L组和40μmol/L组Daoy细胞内β-catenin蛋白水平表达相对吸光度（absorbance

A）值分别为（0.654±0.021）、（0.873±0.034）、（0.869±0.018）、（0.857±0.024）、（0.472±0.035）和（0.351±0.019）(F

浓度×时间

=27.813

P=0.000);VEGF蛋白水平的相对A值为（0.781±0.027）、（0.801±0.025）、（0.792±0.034）、（0.750±0.024）、（0.324±0.015）和（0.201±0.012）(F

浓度

=74.826

P=0.000)。结果还显示

当姜黄素浓度为20μmol/L时

随着药物作用时间的延长

Daoy细胞内β-catenin和VEGF蛋白表达的相对A值明显降低

差异具有统计学意义

呈时间依赖性（F=19.156

P=0.001;F=100.282

P=0.000）。结论:姜黄素可通过下调Wnt/β-catenin通路及下游靶基因VEGF的表达进而抑制Daoy细胞增殖

这或将为姜黄素治疗髓母细胞瘤提供新的理论依据

Abstract

Objective:To observe the effects of curcumin on the growth and vascular growth factors in Daoy cells

and to explore the potential mechanisms. Methods:Human medulloblastoma Daoy cells were cultured in vitro

and treated with different concentrations of curcumin（5

20 and 40 μmol/L） for different times（24

72 h）. MTT assay was used for detecting the effects of curcumin on the proliferation of Daoy cells. ELISA was applied to detect the changes of VEGF levels in the supernatant

and Western blot was used for determining the expression of β-catenin of Wnt/β-catenin pathway and vascular endothelial growth factor（VEGF） at protein levels. Results :MTT results showed that curcumin significantly inhibited the proliferation of Daoy cells

and they were in a concentration-and time-dependent manner. ELISA results demonstrated that curcumin could decrease the generation of VEGF and Western blot indicated that curcumin not only reduced the expression of VEGF

but also reduced the expression of β-catenin

they were also in a concentration-and timedependent manner. Conclusion:Curcumin could inhibit the proliferation of mdulloblastoma Daoy cell

the mechanisms may be related to the inhibitory action of curcumin on Wnt/β-catenin pathway and its downstream VEGF

which will provide a novel theoretical basis for curcumin treating with medulloblastoma.

关键词

姜黄素髓母细胞瘤Wnt/β-catenin血管内皮生长因子

Keywords

curcuminmedulloblastomWnt/β-cateninvascular endothelial growth factor

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