Expression of 14-3-3 family members in HBV-replicated hepatoma carcinoma cells

Zhang Xiang; Wei Jie; Jin Xin; Wang Shilei; Yang Yuan; Shi Yueyuan; Niu Siqiang; Wang Deqiang

doi:10.13406/j.cnki.cyxb.001352

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Expression of 14-3-3 family members in HBV-replicated hepatoma carcinoma cells

更新时间：2024-01-17

- Expression of 14-3-3 family members in HBV-replicated hepatoma carcinoma cells
- Journal of Chongqing Medical University Vol. 42, Issue 7, Pages: 781-786(2017)
- 作者机构：
  
  1. 重庆医科大学检验医学院
  2. 重庆医科大学附属第一医院检验科
  3. 重庆医科大学感染性疾病分子生物学教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001352
  CLC： R512.62;R735.7
- Published：2017，
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Zhang Xiang, Wei Jie, Jin Xin, et al. Expression of 14-3-3 family members in HBV-replicated hepatoma carcinoma cells. [J]. Journal of Chongqing Medical University 42(7):781-786(2017)
DOI：

Zhang Xiang, Wei Jie, Jin Xin, et al. Expression of 14-3-3 family members in HBV-replicated hepatoma carcinoma cells. [J]. Journal of Chongqing Medical University 42(7):781-786(2017) DOI： 10.13406/j.cnki.cyxb.001352.

摘要

目的:研究14-3-3家族在乙型肝炎病毒(hepatitis B virus

HBV)复制的肝癌细胞中的表达。方法:运用q RT-PCR和Western blot分析14-3-3家族在HBV复制细胞系中的表达水平;q RT-PCR、Western blot检测HBV复制在Hep G2细胞中对14-3-3ζ表达的影响;Western blot检测HBV复制在小鼠肝组织中对14-3-3ζ表达的影响。结果:在Hep G2.2.15中

14-3-3η、γ、ζm RNA和蛋白的表达水平较Hep G2对照组明显增加(m RNA:t=16.5、27.86、17.23

P=0.000、0.000、0.000;蛋白:t=35.7、16.97、22.51

P=0.000、0.000、0.000);在Hep AD38(Tet-)中只有14-3-3ζm RNA和蛋白的表达水平较Hep AD38(Tet+)对照组明显增加(m RNA:t=14.27

P=0.000;蛋白:t=12.11

P=0.000);HBV复制能使Hep G2中14-3-3ζm RNA和蛋白的表达显著升高(m RNA:F=98.8

P=0.000;蛋白:F=118.3

P=0.000);HBV复制能使小鼠肝组织中14-3-3ζ蛋白的表达显著升高(F=109.4

P=0.000)。结论:HBV可以促进肝癌细胞内14-3-3η、γ、ζ的表达

这一发现为控制HBV感染的治疗策略提供新的靶点。

Abstract

Objective:To investigate the expression of 14-3-3 family members in HBV-infected hepatocellular carcinoma（HCC）.Methods:The expression levels of 14-3-3 family members in HBV-replicated cell lines were analyzed by q RT-PCR and Western blot. Both q RT-PCR and Western blot were used to detect the effect of HBV replication on the expression of 14-3-3ζ in Hep G2 cells.Western blot was used to investigate the effect of HBV replication on the expression of 14-3-3ζ in mouse liver. Results:The expression levels of 14-3-3η

ζ m RNA and protein in Hep G2.2.15 were significantly higher than those in Hep G2 control group respectively（m RNA:t=16.5

27.86

17.23

P=0.000

0.000

0.000;protein:t=35.7

16.97

22.51

P=0.000

0.000

0.000）. The levels of 14-3-3ζ m RNA and protein in Hep AD38（Tet-）were apparently higher than those in Hep AD38（Tet+）（m RNA:t=14.27

P=0.000;protein:t=12.11

P=0.000）. HBV replication could obviously increase the expression of 14-3-3ζ m RNA and protein in Hep G2（m RNA:F=98.8

P=0.000;protein:F=118.3

P=0.000）. HBV replication could significantly increase the expression of 14-3-3ζ protein in mouse liver tissue. Conclusion:HBV can promote the expression of 14-3-3η

γ and ζ in hepatocarcinoma cells. This finding provides a new target for the treatment strategy for controlling infection of HBV.

关键词

乙型肝炎病毒14-3-3ζ14-3-3家族

Keywords

hepatitis B virus（HBV）14-3-3ζ14-3-3 family members

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