Expression of metabolic pathway in A549 endotracheal orthotopic transplantation lung cancer model

Lu Lei; Wei Siyu; Xu Cai; Lin Xiaojing; He Yunyun; Lian Xuemei

doi:10.13406/j.cnki.cyxb.001346

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Expression of metabolic pathway in A549 endotracheal orthotopic transplantation lung cancer model

更新时间：2024-01-17

- Expression of metabolic pathway in A549 endotracheal orthotopic transplantation lung cancer model
- Journal of Chongqing Medical University Vol. 42, Issue 7, Pages: 888-892(2017)
- 作者机构：
  
  1. 重庆医科大学脂糖代谢性疾病重庆市重点实验室感染性疾病分子生物学教育部重点实验室
  2. 重庆医科大学公共卫生与管理学院医学与社会发展研究中心健康领域社会风险预测治理协同创新中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001346
  CLC： R734.2
- Published：2017，
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Lu Lei, Wei Siyu, Xu Cai, et al. Expression of metabolic pathway in A549 endotracheal orthotopic transplantation lung cancer model. [J]. Journal of Chongqing Medical University 42(7):888-892(2017)
DOI：

Lu Lei, Wei Siyu, Xu Cai, et al. Expression of metabolic pathway in A549 endotracheal orthotopic transplantation lung cancer model. [J]. Journal of Chongqing Medical University 42(7):888-892(2017) DOI： 10.13406/j.cnki.cyxb.001346.

摘要

目的:近年来

代谢与肿瘤发病的病因学研究已经成为热点

同时肺癌仍居恶性肿瘤发病率和死亡率榜首

本研究拟构建一个稳定易操作的肺癌原位移植瘤模型

并初步探讨代谢相关通路在该原位移植瘤模型中的表达。方法:选取雄性6

周龄BABL/c裸鼠10只

优化麻醉用药及手术操作步骤

经气管移植5×106个细胞的A549肺癌细胞成瘤

观察移植瘤形成率、形成部位、大体形态、增殖分化程度。另取4只相同条件裸鼠进行手术空白对照。同时检测代谢相关通路蛋白STAT3、AKT和m TOR的表达情况等。结果:手术最佳麻醉条件为75 mg/kg戊巴比妥钠腹腔注射;改进操作后经气管移植的小鼠肺癌形成率为80.0%;肿瘤形成以右肺上叶居多

呈明显腺癌结构

分化程度较低

移植瘤组织增殖细胞核抗原（proliferating cell nuclear antigen

PCNA）的表达高于肺组织

呈高表达状态。肿瘤负荷肺组织代谢相关通路蛋白信号传导与转录激活因子3（signal transducer and activator of transcription 3

STAT3）、蛋白激酶B（protein kinase B

PKB即AKT）、哺乳动物雷帕霉素靶蛋白（mammalian target of rapamycin

m TOR）的磷酸化水平即p STAT3/STAT3、p AKT/AKT、p-m TOR/m TOR比值均高于正常肺组织

差异具有统计学意义（t=-4.381

-4.462

-4.951;P=0.041

0.011

0.038）。结论:通过改良经气管移植手术方式

可以构建一种操作简单、低成本、高效稳定的A549细胞肺癌移植瘤模型;代谢相关通路在肺癌的发生发展中也发挥了一定作用。

Abstract

Objective:To build a moderate lung cancer orthotopic transplantation tumor model

and to investigate the expression of metabolic pathway in the this model. Methods:After optimizing the dose of anesthetic medicine and operation procedures of endotracheal orthotopic transplantation

5×106 of A549 cells were planted into male nude mice（6-8 weeks old

n=10）lungs. And the blank control（only PBS transplant）was carry out on the same condition mice（n=4）. Then the tumor formation rate

location

anatomy and the expression of signal transducer and activator of transcription 3（STAT3）

protein kinase B（AKT） and mammalian target of rapamycin（m TOR）were studied. Results:The best surgical anaesthesia condition was by intraperitoneal injection of 75 mg/kg body weight pentobarbital sodium. After the improvement of the operation procedure

the total tumor formation rate was 80.0%

and mainly located in the upper lobe of the right lung. The tumor showed a significant glandular cancer structure with poor differentiation degree

and more proliferating cell nuclear antigen（PCAN）-positive cells compared with normal lungs. The levels of phosphorylation of STAT3

AKT and m TOR and the ratio of p STAT3/STAT3

p-AKT/AKT

p-m TOR/m TOR in lung tumors were significantly higher than those in normal lung tissues

the difference was statistically significant differences（t=-4.381

-4.462

-4.951;P=0.041

0.011

0.038 respectively）.Conclusion:The improved endotracheal orthotopic transplanta-tion lung cancer model is a simple

low cost

stable model

and metabolic pathways play a role in the development and progression of lung cancer.

关键词

肺癌A549原位移植信号传导与转录激活因子3蛋白激酶B哺乳动物雷帕霉素靶蛋白

Keywords

lung cancerA549Orthotopic transplantationsignal transducer and activator of transcription 3protein kinase Bmammalian target of rapamycin

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