Role of IL-27 and it’s receptor in the pathogenesis of preterm premature rupture of membrane

Wang Hanbing; Yin Nanlin; Yuan Yu; Qi Hongbo

doi:10.13406/j.cnki.cyxb.001339

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Role of IL-27 and it’s receptor in the pathogenesis of preterm premature rupture of membrane

更新时间：2024-01-17

- Role of IL-27 and it’s receptor in the pathogenesis of preterm premature rupture of membrane
- Journal of Chongqing Medical University Vol. 42, Issue 8, Pages: 1029-1033(2017)
- 作者机构：
  
  重庆医科大学附属第一医院妇产科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001339
  CLC： R714.433
- Published：2017，
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[1]王寒冰,尹楠林,袁雨,漆洪波.IL-27及其受体在未足月胎膜早破发病中的相关研究[J].重庆医科大学学报,2017,42(08):1029-1033.

Wang Hanbing, Yin Nanlin, Yuan Yu, et al. Role of IL-27 and it’s receptor in the pathogenesis of preterm premature rupture of membrane. [J]. Journal of Chongqing Medical University 42(8):1029-1033(2017)
[1]王寒冰,尹楠林,袁雨,漆洪波.IL-27及其受体在未足月胎膜早破发病中的相关研究[J].重庆医科大学学报,2017,42(08):1029-1033. DOI： 10.13406/j.cnki.cyxb.001339.

Wang Hanbing, Yin Nanlin, Yuan Yu, et al. Role of IL-27 and it’s receptor in the pathogenesis of preterm premature rupture of membrane. [J]. Journal of Chongqing Medical University 42(8):1029-1033(2017) DOI： 10.13406/j.cnki.cyxb.001339.

摘要

目的:探讨白介素-27（interleukin-27

IL-27）及其特异性受体WSX-1/TCCR诱导人胎膜组织产生炎性效应在未足月胎膜早破中的作用。方法:（1）将手术室收集到的胎膜组织分为正常足月组和未足月胎膜早破组

将培养的胎膜组织分为对照组、实验组（IL-27处理12 h组以及IL-27处理24 h组）;（2）Western blot和免疫组化分析WSX-1在未足月胎膜早破早产和正常足月孕妇胎膜组织中的表达;（3）培养原代人胎膜组织

鉴定其是否存活;（4）IL-27处理培养的胎膜组织

提取处理的人胎膜组织蛋白

用荧光定量PCR和明胶酶谱检测MMP2、MMP9的表达。结果:（1）IL-27特异性受体WSX-1在未足月胎膜早破早产孕妇胎膜组织中的表达明显高于正常足月孕妇（P=0.0149）;（2）成功培养原代人胎膜组织;（3）人重组IL-27蛋白刺激胎膜组织后MMP2、MMP9的表达明显升高。结论:IL-27可诱导人胎膜组织产生炎性效应

增强MMP2和MMP9的表达

参与未足月胎膜早破早产的发病。

Abstract

Objective:To discuss the role of interleukin-27(IL-27)and its special receptor WSX-1/TCCR in inducing inflammatory effects on human fetal membrane in preterm premature rupture of membrane. Methods:(1)The expression of IL-27 special receptor WSX-1 in fetal membrane of preterm premature rupture of membrane and normal term birth was analyzed by western blot and immunohistochemistry assay.(2)Primary human fetal membrane was isolated and cultured and its survival was identified by RT-PCR.(3)The fetal membranes were divided into control group and the experimental group

treated by 10 ng/m L human recombinant IL-27 and then RNA and protein were extracted. MMP2 and MMP9 m RNA level was detected and their activity was examed by quantitative RT-PCR and Gelatin zymography

respectively. Results:(1)The expression of IL-27 receptor WSX-1 had significant differences between preterm premature rupture of membrane and normal term birth.(2)Primary human fetal membrane was successfully isolated and cultured for processing.(3)Expressions of MMP2 and MMP9 were obviously higher in IL-27 treatment group;P<0.05 was statistically significant. Conclusion:IL-27 could induce human fetal membrane to produce MMP2 and MMP9 in the pathogenesis of preterm premature rupture of membrane.

关键词

未足月胎膜早破早产胎膜组织白介素-27基质金属蛋白酶

Keywords

preterm premature rupture of membraneprimary human fetal membraneinterleukin-27matrix metalloproteinases

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