Expression of HLJ1 in hepatocellular carcinoma cell lines and its biologic function in SK-Hep1

Tan Xixi; Tang Hua

doi:10.13406/j.cnki.cyxb.001325

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Expression of HLJ1 in hepatocellular carcinoma cell lines and its biologic function in SK-Hep1

更新时间：2024-01-17

- Expression of HLJ1 in hepatocellular carcinoma cell lines and its biologic function in SK-Hep1
- Journal of Chongqing Medical University Vol. 42, Issue 7, Pages: 828-833(2017)
- 作者机构：
  
  重庆医科大学感染性疾病分子生物学教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001325
  CLC： R735.7
- Published：2017，
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Tan Xixi, Tang Hua. Expression of HLJ1 in hepatocellular carcinoma cell lines and its biologic function in SK-Hep1. [J]. Journal of Chongqing Medical University 42(7):828-833(2017)
DOI：

Tan Xixi, Tang Hua. Expression of HLJ1 in hepatocellular carcinoma cell lines and its biologic function in SK-Hep1. [J]. Journal of Chongqing Medical University 42(7):828-833(2017) DOI： 10.13406/j.cnki.cyxb.001325.

摘要

目的:研究人肝脏DnaJ-like蛋白(human liver Dna J-like protein

HLJ1)在肝癌细胞中的表达

探讨HLJ1对肝癌细胞SK-Hep1生物学行为的影响。方法:首先

qRT-PCR和Western blot检测HLJ1在肝癌细胞中的表达情况。其次

过表达或干扰HLJ1后转染SK-Hep1细胞株

MTS和克隆形成实验检测细胞增殖能力的变化;流式细胞术检测细胞早期凋亡率;划痕实验检测细胞迁移能力;Transwell小室检测细胞侵袭能力的变化。结果:qRT-PCR和Western blot结果显示HLJ1的mRNA水平和蛋白水平在3种肝癌细胞系中均呈低表达。MTS和克隆形成结果显示HLJ1能抑制肝癌细胞的增殖。流式细胞术结果显示HLJ1能促进细胞早期凋亡。划痕和Transwell实验显示

HLJ1能抑制肝癌细胞的迁移和侵袭。结论:HLJ1在肝癌细胞系中低表达

且能抑制SK-Hep1细胞增殖、迁移和侵袭

促进细胞凋亡。

Abstract

Objective:To investigate the expression of HLJ1 in hepatocellular carcinoma（HCC）cells and to explore the effect of HLJ1 on the biological behavior in SK-Hep1 cells. Methods:The expressions of HLJ1 mRNA and protein were determined by qRT-PCR and Western blot in hepatocellular carcinoma cells（SMMC-7721

SK-Hep1 and HepG2）. MTS

Colony formation and apoptosis assay were performed to determine the cell proliferation and apoptosis;wound healing and Transwell assay were used to detect the migration and invasion in SK-Hep1. Results:qRT-PCR showed HLJ1 m RNA expression were lower in HCC cell lines

and HLJ1 protein level was also lower in HCC cell lines;MTS and Colony formation assay indicated HLJ1 decreased HCC cells proliferation. FCM showed that HLJ1 promoted cell apoptosis. Wound healing and Transwell assay revealed HLJ1 inhibited HCC cell migration. Conclusion:HLJ1 is low expression in HCC cell lines

which could inhibit the proliferation

migration and invasion

while promote cell apoptosis in SK-Hep1.

关键词

肝癌SK-Hep1人肝脏DnaJ-like蛋白生物学行为

Keywords

hepatocellular carcinomaSK-Hep1human liver DnaJ-like proteinbiologic function

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