Effects of different doses of estradiol valerate on intrauterine adhesion in rats

Wang Xian; He Yuanli; Cai Huihua; Li Huijuan; Hu Haiyan; Ren Xuwen; Chen Siping; Zhang Dongmei

doi:10.13406/j.cnki.cyxb.001231

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Effects of different doses of estradiol valerate on intrauterine adhesion in rats

更新时间：2024-01-17

- Effects of different doses of estradiol valerate on intrauterine adhesion in rats
- Journal of Chongqing Medical University Vol. 42, Issue 4, Pages: 378-382(2017)
- 作者机构：
  
  南方医科大学珠江医院妇产科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001231
  CLC： R711.74
- Published：2017，
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Wang Xian, He Yuanli, Cai Huihua, et al. Effects of different doses of estradiol valerate on intrauterine adhesion in rats. [J]. Journal of Chongqing Medical University 42(4):378-382(2017)
DOI：

Wang Xian, He Yuanli, Cai Huihua, et al. Effects of different doses of estradiol valerate on intrauterine adhesion in rats. [J]. Journal of Chongqing Medical University 42(4):378-382(2017) DOI： 10.13406/j.cnki.cyxb.001231.

摘要

目的:研究不同剂量的戊酸雌二醇（estradiol valerate

E2V）对大鼠宫腔粘连（intrauterine adhesions

IUA）的防治效果。方法:将48只8

0周龄健康雌性SD大鼠随机分为8组:空白对照组、实验对照组、3个雌激素预防组（低、中、高剂量）、3个雌激素治疗组（低、中、高剂量）。收集各组大鼠子宫组织

HE染色观察子宫内膜腺体

MASSON染色观察子宫内膜纤维化程度

免疫组化法检测子宫内膜转化生长因子-β1（transforming growth factor-β1

TGF-β1）、基质金属蛋白酶-9（matrix metalloproteinase-9

MMP-9）、雌激素受体-α（estrogen receptor-α

ER-α）的表达水平。结果:与实验对照组相比

3个雌激素预防组大鼠的子宫内膜腺体计数增多（P值均为0.000）、纤维化面积降低(P

低雌A组

=0.013

中雌A组

=0.021

高雌A组

=0.003)

差异均有统计学意义

3个雌激素治疗组大鼠的子宫内膜腺体计数(P

低雌B组

=0.536

中雌B组

=0.536

高雌B组

=1.000)及纤维化面积（P值均为1.000）与实验对照组相比均无显著性差异;6个雌激素组的子宫内膜TGF-β1表达水平均降低（P值均为0.000）

其中中、高剂量组均低于低剂量组（P值分别为0.003及0.002）

而中、高剂量2组相比无差异（P=0.842）;6个雌激素组子宫内膜MMP-9表达水平均升高（P值均为0.000）

中、高剂量组均高于低剂量组（P值均为0.000）

而中、高剂量2组相比无差异（P=0.379）;6个雌激素组子宫内膜ER-α表达水平均有升高（P值均为0.000）

中、高剂量组均高于低剂量组（P值均为0.000）

而中、高剂量2组相比无差异（P=0.513）。结论:在大鼠IUA建模早期应用雌激素

可显示较好疗效

但ER-α饱和后

其疗效不随剂量增加而增强;在建模完成后应用雌激素

治疗效果欠佳

考虑是否与单一治疗有关

如能增加其他治疗方法也许能增强疗效。

Abstract

Objective:To evaluate the prevention and treatment effect of estrodiol valerate on rats intrauterine adhesion. Methods:Forty-eight 8-10 weeks old female SD rats were randomized into 8 groups

6 rats per group:blank control group

experimental control group

three prevention estrogen groups（low dose

medium dose

high dose）

three treatment estrogen groups（low dose

medium dose

high dose）. The uteri were collected. Number of endometrial glands was tested by HE staining;degree of endometrial fobrosis was evaluated by Masson staining

and the endometrial expressions of transforming growth factor-β1（TGF-β1）

matrix metalloproteinase-9（MMP-9）and estrogen receptor-α（ER-α）were detected by immunohistochemistry. Results:In three prevention estrogen groups

endometrial glands increased（the P values were all 0.000）

endometrial fibrosis area ratio decreased(the P values were:P

low group A

=0.013

medium group A

=0.021

high group A

=0.003 respectively)

with statistically significant differences. In three treatment estrogen groups

endometrial glands(P values were:P

low group B

=0.536

medium group B

=0.536

high group B

=1.000)and endometrial fibrosis area ratio（the P values were all 1.000）had no improvement compared with those of experimental control group. TGF-β1 expression level was much lower in 6 estrogen groups than in experimental control group（the P values were all 0. 000）;lower in medium dose and high dose estrogen group than in of low dose estrogen group（the P values were 0.003 and 0.002 respectively）;there was no significant difference between high dose group and medium dose group（P=0.842）. MMP-9 expression level was much higher in 6 estrogen groups than in experimental control group（the P values were 0. 000）

higher in medium dose and high dose estrogen group than in low dose group（P=0.000

P=0.379）. ER-α expression level was much higher in 6 estrogen groups than in experimental control group（the P values were all 0.000）

higher in medium dose and high dose estrogen group than in low dose group（the P values were 0. 000）

there was no significant difference between high dose group and medium dose group（P=0.513）. Conclusion:Estrodiol valerate can prevent rat intrauterine adhesion. The prevention effect is not en hanced with the increase of the dose after the saturation of ER-α. Separate application of E2 V to treat IUA is helpless

comprehensive treatment like TCRA is needed.

关键词

宫腔粘连纤维化戊酸雌二醇雌激素受体-α

Keywords

intrauterine adhesionfibrosisestradiol valerateestrogen receptor-α

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