Establishment of Chinese rural dog intrauterine adhesion model by mechanical and infection double injury

Yang Yuan; Mao Yanhua; Wang Jia; Zhang Yingfeng

doi:10.13406/j.cnki.cyxb.001225

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Establishment of Chinese rural dog intrauterine adhesion model by mechanical and infection double injury

更新时间：2024-01-17

- Establishment of Chinese rural dog intrauterine adhesion model by mechanical and infection double injury
- Journal of Chongqing Medical University Vol. 42, Issue 4, Pages: 401-405(2017)
- 作者机构：
  
  重庆医科大学附属大学城医院妇产科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001225
  CLC： R-332;R711.74
- Published：2017，
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Yang Yuan, Mao Yanhua, Wang Jia, et al. Establishment of Chinese rural dog intrauterine adhesion model by mechanical and infection double injury. [J]. Journal of Chongqing Medical University 42(4):401-405(2017)
DOI：

Yang Yuan, Mao Yanhua, Wang Jia, et al. Establishment of Chinese rural dog intrauterine adhesion model by mechanical and infection double injury. [J]. Journal of Chongqing Medical University 42(4):401-405(2017) DOI： 10.13406/j.cnki.cyxb.001225.

摘要

目的:通过机械和感染双重法构建一个犬的宫腔粘连动物模型

并检测血管内皮因子(vascular endothelial growth factor

VEGF)、转化生长因子(transforming growth factor-β1

TGF-β1)的表达

评估其与宫腔粘连形成的关系

为下一步研究提供实验平台。方法:选取6只性发育成熟的健康雌性中华田园犬

所有建模前的犬的一小段右侧宫角组织作为对照组

建模后该部位子宫角组织作为处理组。利用机械搔刮和感染双重法损伤右侧宫角内膜

于建模前后各收集一小段宫角标本

行HE、Masson染色及SP法免疫组化

整理并统计分析。结果:处理组的内膜厚度[(2.325±0.399)cm]薄于对照组[(3.025±0.466)cm]

差异具有统计学意义(P=0.050);处理组的内膜腺体数目[(4.388±1.774)个]明显比对照组[(16.518±4.421)个]减少

差异具有统计学意义(P=0.001);处理组的子宫内膜纤维化面积比例(0.775±0.040)明显高于对照组(0.329±0.062)

差异有统计学意义(P=0.000);处理组的子宫内膜中VEGF(5.967±0.390)、TGF-β1(6.200±0.306)的表达量高于对照组(1.700±0.823)、(1.100±0.361)

差异均具有统计学意义(P=0.000

P=0.001)。结论:采用机械和感染双重法可以成功构建中华田园犬宫腔粘连的动物模型

且VEGF、TGF-β1的表达量升高可能与宫腔粘连的发生有关。

Abstract

Objective:To establish a stable animal model of intrauterine adhesion by mechanical and infection

to measure the expressions of vascular endothelial growth factor（VEGF） and transforming growth factor-β1（TGF-β1） and to evaluate their relationship with the formation of adhesion

in order to provide a experimental platform for the next research. Methods:The uterus from 6 healthy mature female Chinese pastoral dogs were collected and divided into two groups:the treatment group and the control group. The right uterine endometriums were collected and evaluated using HE staining

Masson’s stain and SP immunohistochemistry. Results:The endometrial thickness of treatment group（2.325±0.399）was thinner than that（3.025±0.466）of control group;the number of endometrial glands of treatment group（4.388±1.774）was significantly fewer than that（16.518±4.421）of control group;the endometrial fibrosis area（0.775 ±0.040） was significantly higher than that（0.329 ±0.062） of control group. The expression levels of VEGF（5.967±0.390）and TGF-β1（6.200±0.306）in endometrium of treatment group were significantly higher than those（1.700±0.823）and（1.100±0.361）in the control group. Conclusion:The animal model of intrauterine adhesions are constructed successfully by mechanical and infective method. The high expressions of VEGF and TGF-β1 may be closely related with the occurrence of intrauterine adhesion.

关键词

宫腔粘连动物模型损伤感染犬科

Keywords

intrauterine adhesiousanimal modelinjuryinfectioncanidae

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