Steroid receptor coactivator-3 is involved in the pathogenesis of preeclampsia by regulating PI3K/Akt/mTOR pathway

Yuan Yu; Shan Nan; Tan Bin; Liu Yangming; He Chengjin; Qi Hongbo

doi:10.13406/j.cnki.cyxb.001209

您当前的位置：

首页 >

文章列表页 >

Steroid receptor coactivator-3 is involved in the pathogenesis of preeclampsia by regulating PI3K/Akt/mTOR pathway

更新时间：2024-01-17

- Steroid receptor coactivator-3 is involved in the pathogenesis of preeclampsia by regulating PI3K/Akt/mTOR pathway
- Journal of Chongqing Medical University Vol. 42, Issue 8, Pages: 1034-1038(2017)
- 作者机构：
  
  重庆医科大学附属第一医院妇产科重庆医科大学"中国-加拿大-新西兰"联合母胎医学实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001209
  CLC： R714.244
- Published：2017，
扫描看全文
[1]袁雨,单楠,谭彬,刘洋铭,何承晋,漆洪波.类固醇受体辅助活化因子-3通过PI3K/Akt/mTOR信号通路参与子痫前期发病机制中的作用研究[J].重庆医科大学学报,2017,42(08):1034-1038.

Yuan Yu, Shan Nan, Tan Bin, et al. Steroid receptor coactivator-3 is involved in the pathogenesis of preeclampsia by regulating PI3K/Akt/mTOR pathway. [J]. Journal of Chongqing Medical University 42(8):1034-1038(2017)
[1]袁雨,单楠,谭彬,刘洋铭,何承晋,漆洪波.类固醇受体辅助活化因子-3通过PI3K/Akt/mTOR信号通路参与子痫前期发病机制中的作用研究[J].重庆医科大学学报,2017,42(08):1034-1038. DOI： 10.13406/j.cnki.cyxb.001209.

Yuan Yu, Shan Nan, Tan Bin, et al. Steroid receptor coactivator-3 is involved in the pathogenesis of preeclampsia by regulating PI3K/Akt/mTOR pathway. [J]. Journal of Chongqing Medical University 42(8):1034-1038(2017) DOI： 10.13406/j.cnki.cyxb.001209.

摘要

目的:探讨类固醇受体辅助活化因子-3（steroid receptor coactivator-3

SRC-3）参与人脐静脉内皮细胞株（HUVECs）受损的信号通路

及其在子痫前期发病机制中的作用。方法:收集2014年10月至2015年10月在重庆医科大学附属第一医院行选择性剖宫产手术的正常足月妊娠组（31例）以及子痫前期组（29例）的胎盘组织。使用Western blot检测2组胎盘组织SRC-3的表达。脐静脉内皮细胞株（HUVECs）分为4个处理组:正常常氧对照（N）培养组、缺氧/复氧（H/R）培养组、SRC-3 sh RNA慢病毒敲降（sh SRC-3）培养组以及PI3K特异性抑制剂（LY294002）培养组。使用Western blot检测内皮细胞中SRC-3、p-Akt（Ser473）、Akt、p-m TOR（Ser2481）、m TOR的表达。使用细胞管腔成型实验检测内皮细胞血管形成能力。结果:SRC-3在子痫前期胎盘组织蛋白水平低于正常足月胎盘（t=3.501

P=0.013）。细胞学实验中

与N组相比

H/R组、sh SRC-3组及LY294002组HUVECs的SRC-3（t=4.236

P=0.007;t=5.469

P=0.002;t=4.165

P=0.008）、p-Akt（Ser473）/Akt（t=5.214

P=0.002;t=5.188

P=0.002;t=7.054

P=0.000）、p-m TOR（Ser2481）/m TOR（t=4.832

P=0.003;t=5.695

P=0.001;t=5.699

P=0.001）表达降低。H/R组、sh SRC-3组、LY294002组HUVECs血管形成能力低于N组（t=5.268

P=0.002;t=4.648

P=0.004;t=4.087

P=0.009）。结论:SRC-3可能通过PI3K/Akt/m TOR通路参与子痫前期的发病机制。

Abstract

Objective:To explore the mechanism of steroid receptor coactivator-3(SRC-3)involved in in the pathogenesis of preeclampsia(PE). Methods:Western blot was used to detect the expression of SRC-3 protein in placentas. Cell treatments of each group were as follows:normal culture group(N)

hypoxia/reoxygenation(H/R) culture group

lentivirus short hairpin RNA against SRC-3transfect group(sh SRC-3)

PI3 K inhibitor LY294002 culture group(LY294002). Western blot was used to detect the expression of SRC-3

p-Akt(Ser473)

Akt

p-m TOR(Ser2481)

m TOR protein in HUVECs. The tube formation assay was used to detect tube formation capacity of HUVECs. Results:The protein levels of SRC-3 were decreased in PE placentas than in normal placentas(t =3.501

P =0.013). In four cell groups

the expression of SRC-3(t=4.236

P=0.007;t=5.469

P=0.002;t=4.165

P=0.008)

p-Akt(Ser473)/Akt(t=5.214

P =0.002;t =5.188

P =0.002;t =7.054

P =0.000)

p-m TOR(Ser2481)/m TOR(t =4.832

P =0.003;t =5.695

P =0.001;t =5.699

P =0.001)proteins in H/R group

sh SRC-3 group and LY294002 group were lower than that in normal group. Moreover

the tube formation ability of H/R group

sh SRC-3 group and LY294002 group were reduced(t=5.268

P=0.002;t=4.648

P=0.004;t=4.087

P=0.009).Conclusion:These results suggest that SRC-3 may play a critical role in the pathogenesis of PE though PI3K/Akt/m TOR pathway.

关键词

子痫前期类固醇受体辅助活化因子-3血管内皮PI3K/Akt/m TOR

Keywords

preeclampsiasteroid receptor coactivator-3human umbilical vein endothelial cellsPI3K/Akt/mTOR

references

Views

193

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Application value of placental growth factor，soluble fms-like tyrosine kinase-1，and glycosylated fibronectin in the prediction of preeclampsia

Study on the diagnostic value of inflammation score system in preeclampsia

Effect of GPR30 protein on function of trophoblast cells and its relationship with the development of preeclampsia

Relationship between expression of autophagy regulation interleukin-33 and the pathogenesis of preeclampsia

Effects of MEST/PEG1 on cell migration and invasion in the human trophoblast cells and its roles in preeclampsia

Related Author

Tang Ye

Zhao Li

Zhao Di

Su Jingna

Shi Hao

Xiao Jianping

Yang Lan

Cai Yu

Related Institution

Department of Medical Genetics and Prenatal Diagnosis，Wuxi Maternal and Child Health Hospital

Department of Obstetrics and Gynecology，Third Affiliated Hospital of Zunyi Medical University， Zunyi First People’s Hospital

Department of Obstetrics and Gynecology,The First Affiliated Hospital of Chongqing Medical University

Department of Emergency and Intensive Care Unit,The First Affiliated Hospital of Chongqing Medical University

Laboratory of Reproductive Biology,School of Public Health and Management,Chongqing Medical University,Canada-China-New Zealand Joint Laboratory of Maternal and Fetal Medicine of Chongqing Medical University

⁰