Running exercise reduces dendritic spine loss in the dentate gyrusof the hippocampus in middle-aged APP/PS1 transgenic AD mice

Tang Wei; Chao Fenglei; Jiang Lin; Zhang Yi; Zhou Chunni; Zhang Lei; Liang Xin; Zhu Yinqing; Huang Wei; Li Changqiong; Li Tao; Zhao Zhenhua; Tang Yong

doi:10.13406/j.cnki.cyxb.001184

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Running exercise reduces dendritic spine loss in the dentate gyrusof the hippocampus in middle-aged APP/PS1 transgenic AD mice

更新时间：2024-01-17

- Running exercise reduces dendritic spine loss in the dentate gyrusof the hippocampus in middle-aged APP/PS1 transgenic AD mice
- Journal of Chongqing Medical University Vol. 42, Issue 6, Pages: 707-712(2017)
- 作者机构：
  
  重庆医科大学基础医学院人体解剖与组织胚胎学教研室干细胞与组织工程研究室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001184
  CLC： R749.16
- Published：2017，
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Tang Wei, Chao Fenglei, Jiang Lin, et al. Running exercise reduces dendritic spine loss in the dentate gyrusof the hippocampus in middle-aged APP/PS1 transgenic AD mice. [J]. Journal of Chongqing Medical University 42(6):707-712(2017)
DOI：

Tang Wei, Chao Fenglei, Jiang Lin, et al. Running exercise reduces dendritic spine loss in the dentate gyrusof the hippocampus in middle-aged APP/PS1 transgenic AD mice. [J]. Journal of Chongqing Medical University 42(6):707-712(2017) DOI： 10.13406/j.cnki.cyxb.001184.

摘要

目的:运用现代体视学方法定量研究跑步锻炼对APP/PS1双转基因模型小鼠海马齿状回树突棘的作用。方法:12月龄APP/PS1双转基因阿尔茨海默病(Alzheimer’s disease

AD)模型小鼠随机分为跑步组和阳性对照组

同窝生野生型小鼠作为阴性对照组

每组各15只。跑步组小鼠进行4个月的跑步锻炼。运用Morris水迷宫方法测试3组小鼠的空间学习记忆能力

现代体视学方法定量研究3组小鼠海马齿状回树突棘密度和总数。结果:定位航行实验中跑步组小鼠逃避潜伏期[(25.0±2.5)s]明显短于阳性对照组小鼠[(35.4±2.5)s](P=0.016)。空间探索试验中跑步组小鼠穿台次数、目标象限游泳时间、目标象限游泳距离百分比[(3.2±1.9)次、(21.5±7.6)s、(0.31±0.12)%]明显多于阳性对照组小鼠[(1.3±0.9)次、(14.0±8.2)s、(0.23±0.14)%](P=0.01

P=0.029

P=0.019);跑步组小鼠海马齿状回内树突棘密度、总数[(0.431 9±0.044 8)/μm3、(4.546 6±1.081 4)×108]明显高于阳性对照组[(0.285 6±0.013 3)/μm3、(2.667 9±0.643 5)×108](P<0.001

P=0.043)。结论:跑步锻炼对AD小鼠海马齿状回树突棘的作用可能是跑步锻炼改善AD小鼠空间学习能力的重要结构基础之一。

Abstract

Objective:To investigate the effect of running excise on dendritic spines in the dentate gyrus（DG）of the hippocampus in middle-aged male APP/PS1 transgenic Alzheimer’s disease（AD）mice using the unbiased stereological methods. Methods:Twelvemonth-old male APP/PS1 transgenic AD mice were randomly assigned to a running group or a positive control group. Meanwhile

agematched male wild-type mice were used as a negative control group（n=15 per group）. The mice of the running group were placed on a treadmill to run for 4 months. The spatial learning and memory abilities were investigated using the Morris water maze test. The density and the total number of the dendritic spines in the DG of the mice in three groups were investigated using the unbiased stereological methods. Results:In the hidden platform task

the escape latency of the running exercise group mice（25.0±2.5）s was significantly shorter than that of the positive control group mice（35.4±2.5）s（P=0.016）. In the probe trial task

the times of platform location crosses

the time spending in the target quadrant（s）and swimming distance percentage in the target quadrant of running group [（3.2 ±1.9）

（21.5 ±7.6） s

（0.31 ±0.12）] were significantly more than positive control group [（1.3 ±0.9）

（14.0 ±8.2） s

（0.23±0.14）]（P=0.01

P=0.029

P=0.019）. The density and the total number of the dendritic spines in the running group [（0.431 9±0.044 8）/μm3

（4.546 6±1.081 4）×108] were significantly increased compared to those of the positive control group[（0.2856 ±0.013 3）/μm3

（2.667 9 ±0.643 5）×108]（P <0.001

P=0.043）. Conclusion:The positive effects of running exercise on the spatial learning and memory abilitiesof APP/PS1 mice might be related to the protective effects ofrunning exercise on the dendritic spines in the DG.

关键词

树突棘齿状回APP/PS1 AD小鼠体视学

Keywords

dendritic spinedentate gyrusAPP/PS1 transgenetic micestereology

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