Objective:To evaluate the effects of sperm with different qualities on the outcomes of intracytoplasmic sperm injection(ICSI). Methods:The clinical data of patients received ICSI treatment with different sources and parameters of semen were retrospectively analyzed. Group A were patients who received 228 cycles due to sperm-egg combination obstacles. Group B were patients who received 790 cycles due to oligo-asthenospermatism. Group C were patients who received percutaneous epididymal aspiration(PESA)/testicular sperm extraction(TESA) for 225 cycles. The embryonic development and pregnancy outcomes were compared. Results:There was no statistical difference in rate of fertilization rate and cleavage among three group. The rate of available embryo of group A was lower than that in group B;the rate of good-quality embryo of group A is lower than that in group B and C;group A has a significantly lower implantation rate than group B and C;there was no significant difference in clinical pregnancy rate

rate of multiplets

early miscarriage

premature birth rate

live birth rate and newborn sex ratio among three groups in the fresh transplantation cycles(P >0.05). The neonatal weight of group A was higher than that of groups B and C

while the neonatal weight of group B is higher than that of group C. In the vitrified-thawed cycles

the clinical pregnancy rate and the implantation rate of group A were lower than those of group B and group C

and the clinical pregnancy rate and the implantation rate of group C were higher than those of group B;group C has a higher live-birth rate than group A and group B;there was no statistical difference in other indexes. Conclusion:The parameters and source of sperm does not affect the ICSI fertilization

cleavage and early pregnancy. ICSI can improve the fertilization rate of sperm-egg combination obstacles(group A)

but the quality and developmental potential of embryos is lower. PESA/TESA groups have a relatively higher clinical pregnancy and implanation rate.