Effect of extracorporeal shock wave with Chuan-zhi Plaster on expression of interleukin-1β and tumor necrosisfactor-α in cartilage in rabbits with knee osteoarthritis

Deng Yao

doi:10.13406/j.cnki.cyxb.001132

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Effect of extracorporeal shock wave with Chuan-zhi Plaster on expression of interleukin-1β and tumor necrosisfactor-α in cartilage in rabbits with knee osteoarthritis

更新时间：2024-01-17

- Effect of extracorporeal shock wave with Chuan-zhi Plaster on expression of interleukin-1β and tumor necrosisfactor-α in cartilage in rabbits with knee osteoarthritis
- Journal of Chongqing Medical University Vol. 42, Issue 2, Pages: 130-133(2017)
- 作者机构：
  
  胜利油田中心医院风湿免疫科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001132
  CLC： R684.3
- Published：2017，
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Deng Yao. Effect of extracorporeal shock wave with Chuan-zhi Plaster on expression of interleukin-1β and tumor necrosisfactor-α in cartilage in rabbits with knee osteoarthritis. [J]. Journal of Chongqing Medical University 42(2):130-133(2017)
DOI：

Deng Yao. Effect of extracorporeal shock wave with Chuan-zhi Plaster on expression of interleukin-1β and tumor necrosisfactor-α in cartilage in rabbits with knee osteoarthritis. [J]. Journal of Chongqing Medical University 42(2):130-133(2017) DOI： 10.13406/j.cnki.cyxb.001132.

摘要

目的:探讨体外冲击波(extracorporeal shock wave

ESW)配合穿蛭膏对家兔膝骨关节炎白细胞介素-1β(interleukin-1β

IL-1β)及肿瘤坏死因子-α(tumour necrosis factor-α

TNF-α)表达的影响。方法:将48只健康新西兰大白兔随机分为对照组、模型组、穿蛭膏治疗组(研究组A)、ESW+穿蛭膏治疗组(研究组B)

每组各12只。除对照组外的大白兔采用Hulth法制备骨关节炎模型。研究组A在造模后立即给予穿蛭膏外敷

3次/d;研究组B在造模后立即给予ESW配合穿蛭膏外敷于膝关节治疗

ESW治疗的能流密度为0.1 m J/mm2

冲击次数为1 000次

穿蛭膏外敷3次/d。对照组不做任何处理。处理1个月后

将4组大白兔处死。观察关节软骨大体标本形态学变化

并采用免疫组织化学法和ELISA法检测4组大白兔关节软骨以及关节液中IL-1β和TNF-α的水平。结果:模型组、研究组A和研究组B家兔关节软骨及关节液内的IL-1β和TNF-α水平均较对照组上升(P<0.05)

研究组B的水平较研究组A和模型组均下降(P<0.05)。结论:ESW配合穿蛭膏能降低家兔OA中IL-1β、TNF-α的表达。

Abstract

Objective:To explore the effect of extracoporeal shock wave（ESW）with Chuan-zhi plaster on expression of interleukin-1β（IL-1β）and tumor necrosis factor-α（TNF-α）in cartilage in rabbits with knee osteoarthritis. Methods:Totally 48 rabbits were randomly divided into control group

model group

treatment A group and treatment B group

with 12 rabbits in each group. Model of knee osteoarthritis was established in model group

treatment A group and treatment B group with Hulth method. Rabbits in treatment A group was treated with Chuan-zhi plaster

those in treatment B group was treated with ESW（each 1 000 impulse

the energy flux density of 0.1 m J/mm2）and Chuan-zhi plaster. The rabbits were sacrificed respectively at 4 weeks after ESWT. The articular genurinse solutions were collected from rats;the expressions of IL-1β and TNF-α in synovia were detected by ELISA

and immunohistochemical staining was used to observe the expression characteristics of IL-1β and TNF-α in cartilage of each group;differences were compared among the groups. Results:The expressions of IL-1β and TNF-α were significantly lower in treatment B group than in the model group and treatment A group（P<0.01）. Conclusion:ESW with Chuan-zhi plaster can down-regulate the expression of IL-1β and TNF-α in chondrocytes with osteoarthritis.

关键词

膝骨关节炎体外冲击波穿蛭膏白细胞介素-1β肿瘤坏死因子-α

Keywords

osteoarthritisextracoporeal shock waveChuan-zhi plasterinterleukin-1βtumor necrosis factor-α

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