Objective:To investigate the changes of the learning of trace eyeblink conditioning（TEBC）and prefrontal cortex（PFC）’s event-related potential in senescence accelerated mouse-prone/8（SAM-P8）mice. Methods:Experimental animals were divided into SAM-P8 group（n=11）and SAM-R1 control group（n=11）;two groups received conditioned stimulus（CS）and unconditioned stimulus（US）paired training

in which

CS is a 250 ms

3 k Hz

85 d B tone and US is a 3.0 Psi

100 ms corneal air puff. Then the CR acquirement of TEBC in the two groups were observed

and changes of PFC’s event-related potential during this process were compared between the two groups. Results:Results behavioral results:SAM-P8 mice acquired limited classical TEBC with optimal CS（250 ms

3k Hz

85 d B tone）;the CR% of SAM-R1 was higher than that of SAM-P8（P<0.05）. In PFC-ERP:there are no significant difference in peak-peak amplitude or latency of peak between SAM-P8 group and SAM-R1 group

and there was also no significant difference in training periods between two groups（P>0.05）in CR period. In pre-CR period:peak-peak amplitude of PFC-ERP in SAM-P8 mice reduced observably compared with that of SAM-R1（P<0.05）;peak-peak amplitude of PFC-ERP in medium-term of behavior training was much higher than that in early period of training in SAM-R1 mice（P<0.05）

but no related changes in SAM-P8 were observed（P>0.05）. Conclusion:SAM-P8’s learning capability of TEBC has been impaired but the impairment is incomplete. During the establishment of TEBC

PFC involvement of SAM-P8 mice is less than that of SAM-R1 mice;PFC may be more involved during the medium-term of behavior training

but this time characteristic is not obvious in SAM-P8.