Application of CRISPR/Cas9 technology in hepatitis B virus infection

Luo Yingying; Huang Ailong; Hu Jieli

doi:10.13406/j.cnki.cyxb.001111

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Application of CRISPR/Cas9 technology in hepatitis B virus infection

更新时间：2024-01-17

- Application of CRISPR/Cas9 technology in hepatitis B virus infection
- Journal of Chongqing Medical University Vol. 42, Issue 7, Pages: 905-909(2017)
- 作者机构：
  
  重庆医科大学感染性疾病分子生物学教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001111
  CLC： R512.62
- Published：2017，
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Luo Yingying, Huang Ailong, Hu Jieli. Application of CRISPR/Cas9 technology in hepatitis B virus infection. [J]. Journal of Chongqing Medical University 42(7):905-909(2017)
DOI：

Luo Yingying, Huang Ailong, Hu Jieli. Application of CRISPR/Cas9 technology in hepatitis B virus infection. [J]. Journal of Chongqing Medical University 42(7):905-909(2017) DOI： 10.13406/j.cnki.cyxb.001111.

摘要

CRISPR/Cas9基因剪辑技术源于细菌及古细菌CRISPR介导的后天获得性免疫系统

通过引导RNA与靶DNA PAM序列特异性识别

引发Cas9核酸酶定点切割互补双链DNA

在基因剪辑方面具有制备简单、剪辑位点特异性高及易于同时剪辑多个基因位点等优势

已被广泛应用于哺乳动物细胞基因剪辑。本文主要从CRISPR/Cas9技术作用原理和其抗乙型肝炎病毒感染作用及机制等方面进行综述。

Abstract

The clustered regularly interspaced short palindromic repeats（CRISPR）/CRISPR-associated（Cas）9 system is an adaptive immune system. The sg RNA guides the Cas9 protein to specifically cut target DNA sequence by recognizing the protospacer-adjacent motif（PAM） sequence in target DNA. CRISPR/Cas9 offers several potential advantages

including the ease of customization

higher targeting efficiency and the ability to facilitate multiplex geno-me editing. Due to these advantages

it has been used in gene editing in several kinds of mammalian cells. In this review

we discuss the mechanism of CRISPR/Cas9

application of CRISPR/Cas9 in hepatitis B virus infection and relevant mechanism.

关键词

CRISPR基因剪辑乙型肝炎病毒共价闭合环状DNA

Keywords

clustered regularly interspaced short palindromic repeatsgene editinghepatitis B viruscccDNA

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Related Author

Lan Yukang

Qi Xiaoying

Gui Xien

Zhuang Ke

Huang Yao

Luo Xuan

Chen Yanmeng

Huang Ailong

Related Institution

Medical College of Wuhan University of Science and Technology

Department of Infectious Diseases， Puren Hospital Affiliated to Wuhan University of Science and Technology

Department of Infectious Diseases， Zhongnan Hospital of Wuhan University

Center for Animal Experiment/Animal Biosafety Level-III Laboratory， Wuhan University

Key Laboratory of Molecular Biology on Infectious Disease,Ministry of Education,Chongqing Medical University

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