Changes in expression of Aquaporin-9 in the hippocampus of APP/PS1 double transgenic mouse model of Alzheimer’s disease

Lu Weitian; Huang Juan; Li Yu; Sun Shanquan

doi:10.13406/j.cnki.cyxb.001103

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Changes in expression of Aquaporin-9 in the hippocampus of APP/PS1 double transgenic mouse model of Alzheimer’s disease

更新时间：2024-01-17

- Changes in expression of Aquaporin-9 in the hippocampus of APP/PS1 double transgenic mouse model of Alzheimer’s disease
- Journal of Chongqing Medical University Vol. 42, Issue 1, Pages: 120-124(2017)
- 作者机构：
  
  重庆医科大学神经科学研究中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001103
  CLC： R749.16
- Published：2017，
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[1]陆蔚天,黄娟,李昱,孙善全.AQP9在APP/PS1双转基因小鼠海马中的表达变化[J].重庆医科大学学报,2017,42(01):120-124.

Lu Weitian, Huang Juan, Li Yu, et al. Changes in expression of Aquaporin-9 in the hippocampus of APP/PS1 double transgenic mouse model of Alzheimer’s disease. [J]. Journal of Chongqing Medical University 42(1):120-124(2017)
[1]陆蔚天,黄娟,李昱,孙善全.AQP9在APP/PS1双转基因小鼠海马中的表达变化[J].重庆医科大学学报,2017,42(01):120-124. DOI： 10.13406/j.cnki.cyxb.001103.

Lu Weitian, Huang Juan, Li Yu, et al. Changes in expression of Aquaporin-9 in the hippocampus of APP/PS1 double transgenic mouse model of Alzheimer’s disease. [J]. Journal of Chongqing Medical University 42(1):120-124(2017) DOI： 10.13406/j.cnki.cyxb.001103.

摘要

目的:研究水通道蛋白9（aquaporin-9

AQP9）在阿尔茨海默病（Alzheimer’s disease

AD）模型小鼠海马中的表达变化。方法:9月龄APPswe/PS1d E9双转基因雄性小鼠和C57野生型雄性小鼠各12只

分别为AD模型组和对照组

采用水迷宫实验检测2组小鼠学习记忆能力

采用免疫组化、免疫印迹和q RT-PCR方法检测AQP9蛋白及其m RNA在以上2种小鼠海马中的表达。结果:水迷宫实验显示

与对照组比较

模型组小鼠找到平台的潜伏期显著加长

穿越平台次数明显减少（t=7.064

P=0.000）。免疫组化、免疫印迹和q RT-PCR结果分别显示:与对照组比较

AQP9的吸光度（absorbance

A）值在海马不同区域均增加（CA1:t=7.373

P=0.000;CA2:t=3.1

P=0.011;CA3:t=4.428

P=0.001;齿状回:t=5.285

P=0.000）;AQP9蛋白表达增高（t=5.305

P=0.003）;AQP9 m RNA表达水平增高（t=3.646

P=0.045）。结论:在APPswe/PS1d E9双转基因AD模型小鼠海马中

AQP9表达上调。

Abstract

Objective:To investigate the changes in expression of Aquaporin-9(AQP9) in the hippocampus of mouse model of Alzheimer’s disease(AD). Methods:APPswe/PS1 d E9 double transgenic male mice aged 9 months(n=12)and C57 wild-type(WT)male mice aged 9 months(n=12)were used as model of AD and control

respectively. Morris water maze tests were used to assess the learning and memory abilities of animals. Immunohistochemical staining

Western blot and q RT-PCR were applied to exam the expression of AQP9 protein and its m RNA in the hippocampus of the AD model and WT mice. Results:The water maze test showed that AD model mice took longer latency and had significantly less platform-passing times than WT mice(t=7.064

P=0.000). The results of immunohistochemical staining showed that the absorbance(A)value of AQP9 immunoreactivity in different hippocampus areas were increased in the hippocampus of the AD model mice compared with in that of the WT mice(CA1:t=7.373

P=0.000;CA2:t=3.1

P=0.011;CA3:t=4.428

P=0.001;girus:t=5.285

P=0.000). The results of Western blot showed that the expression of AQP9 protein were increased compared with in that of the WT mice(t=5.305

P=0.003). The q RT-PCR showed that AQP9 m RNA were increased in the hippocampus of the AD model mice compared with in that of the WT mice(t=3.646

P=0.045). Conclusion:AQP9 protein and its m RNA are upregulated in the hippocampus of the APPswe/PS1 d E9 double transgenic mice model of AD.

关键词

水通道蛋白9阿尔茨海默病APPswe/PS1d E9转基因小鼠海马

Keywords

aquaporin-9Alzheimer’s diseaseAPPswe/PS1dDE9 double transgenic micehippocampus

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