Objectives:To investigate the effects of dai zong fang（DZF）on glucose uptake and glucose transporter 4 protein expression in skeletal muscle cells. Methods:C2 C12 skeletal muscle cells were differentiated into myotubes by incubating in DMEM with 2%horse serum;glucose uptake in C2 C12 myotubes were measured by 2-NBDG;the dose effect and time effect of 2-NBDG were analyzed. After the intervention of different concentrations of DZF

glucose uptake in C2 C12 myotubes were analyzed in basal and insulin-stimulated state. Effects of DZF on GLUT4 protein expression and translocation were analyzed by Western blot. Results:Both in basal and insulin-stimulated state

different concentrations of DZF could stimulate the glucose uptake in skeletal muscle cells

with statistically significant difference（P<0.05）. In the basal state

DZF significantly increased total and membrane GLUT4 protein expression（P<0.05）. In the insulin-stimulated state

DZF had no significant effect on the total GLUT4 protein expression（P>0.05）

however

it could significantly promote the membrane GLUT4 protein expression（P<0.05）. Conclusion:DZF could increase glucose uptake in skeletal muscle cells and have insulin-like function

the mechanisms might be related to the promotion of GLUT4 translocation.