Stomatin-like protein 2(SLP-2) regulates cell proliferation and differenation in endometrial stromal cell line T-HESC

Feng Qian; Liu Xueqing; Zhang Xue; Lan Xi; Geng Yanqing; Chen Xuemei; Gao Rufei; He Junlin; Wang Yingxiong; Ding Yubin

doi:10.13406/j.cnki.cyxb.001069

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Stomatin-like protein 2(SLP-2) regulates cell proliferation and differenation in endometrial stromal cell line T-HESC

更新时间：2024-01-17

- Stomatin-like protein 2(SLP-2) regulates cell proliferation and differenation in endometrial stromal cell line T-HESC
- Journal of Chongqing Medical University Vol. 42, Issue 8, Pages: 936-941(2017)
- 作者机构：
  
  重庆医科大学公共卫生与管理学院生殖生物学实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001069
  CLC： R711.6
- Published：2017，
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[1]冯倩,刘学庆,张雪,兰曦,耿艳清,陈雪梅,高茹菲,何俊琳,王应雄,丁裕斌.SLP-2调控人子宫内膜基质细胞细胞系T-HESC的增殖和分化[J].重庆医科大学学报,2017,42(08):936-941.

Feng Qian, Liu Xueqing, Zhang Xue, et al. Stomatin-like protein 2(SLP-2) regulates cell proliferation and differenation in endometrial stromal cell line T-HESC. [J]. Journal of Chongqing Medical University 42(8):936-941(2017)
[1]冯倩,刘学庆,张雪,兰曦,耿艳清,陈雪梅,高茹菲,何俊琳,王应雄,丁裕斌.SLP-2调控人子宫内膜基质细胞细胞系T-HESC的增殖和分化[J].重庆医科大学学报,2017,42(08):936-941. DOI： 10.13406/j.cnki.cyxb.001069.

Feng Qian, Liu Xueqing, Zhang Xue, et al. Stomatin-like protein 2(SLP-2) regulates cell proliferation and differenation in endometrial stromal cell line T-HESC. [J]. Journal of Chongqing Medical University 42(8):936-941(2017) DOI： 10.13406/j.cnki.cyxb.001069.

摘要

目的:研究stomatin样蛋白2（stomatin-like protein 2

SLP-2）对人子宫内膜基质细胞系T-HESC的增殖和分化的调控作用。方法:培养子宫内膜基质细胞系T-HESC

体外诱导蜕膜化

RT-q PCR、Western blot和免疫荧光方法检测SLP-2在体外诱导蜕膜化模型中的表达变化以及蜕膜化标志分子催乳素（prolactin

PRL）、胰岛素样生长因子结合蛋白1（insulin like growth factor binding protein 1

IGFBP1）和Desmin的表达。转染特异性SLP-2干扰小RNA（si RNA）

敲低SLP-2的表达

分析敲低SLP-2表达对基质细胞增殖和分化（蜕膜化）的影响。结果:体外诱导蜕膜化条件下

诱导蜕膜化标志物IGFBP1和PRL的表达明显增加;Desmin显著表达于在体外诱导蜕膜化的细胞中;SLP-2表达明显升高。在蜕膜化细胞中转染SLP-2 si RNA敲低SLP-2表达

蜕膜化行为受到显著抑制;细胞增殖标志分子CCND3表达明显下降

细胞增殖降低。结论:SLP-2的表达可影响人子宫内膜基质细胞细胞系T-HESC的增殖和分化。

Abstract

Objective:To study the role of stomatin-like protein 2(SLP-2)in regulating proliferation and differentiation in humans endometrial stromal cell line T-HESC. Methods:The cultured human endometrial stromal cell line T-HESC was used an in vitro model to test the expression of SLP-2 and decidualization marker molecules insulin like growth factor binding protein 1(IGFBP1)

prolactin(PRL)and Desmin in induced decidualized T-HESC by RT-q PCR

Western blot and Immunofluorescence. Small inte-rference RNA(si RNA)was used to knock down of SLP-2 and study the role of SLP-2 in T-HESC cell proliferation and differentiation under decidualization induction. Results :The expression of decidualization marker IGFBP1

PRL and Desmin was significantly increased under induced decidualization in vitro. SLP-2 expression was stimulated in decidualized T-HESC. Knocking down SLP-2by si RNA in decidualized T-HESC critically decreased T-HESC cell proliferation and downregulated proliferation makers Ed U and CCND3. The expression of IGFBP1 and PRL was also significantly reduced following SLP-2 si RNA transfection in decidulized THESC. Conclusion:SLP-2 is involved in regulating T-HESC cell proliferation and differentiation.

关键词

Stomatin样蛋白2胚胎植入蜕膜化子宫内膜基质细胞

Keywords

stomatin-like protein 2(SLP-2)embryo implantationdecidualizationendothelial stromal cell

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Laboratory of the Reproductive Biology, School of Public Health and Management,Chongqing Medical University

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Laboratory of Reproductive Biology,School of Public Health and Management,Chongqing Medical University

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