Proliferation and migration ability of tongue squamous cells before and after exposure to cisplatin

Wang Jun; Ji Ping; Li Lifeng; Gao Lina; Guan Chao; Su Kexin; Li Linlin

doi:10.13406/j.cnki.cyxb.001034

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Proliferation and migration ability of tongue squamous cells before and after exposure to cisplatin

更新时间：2024-01-17

- Proliferation and migration ability of tongue squamous cells before and after exposure to cisplatin
- Journal of Chongqing Medical University Vol. 41, Issue 10, Pages: 1027-1032(2016)
- 作者机构：
  
  1. 重庆医科大学附属口腔医院颌面外科
  2. 重庆医科大学附属口腔医院口腔疾病与生物医学重庆市重点实验室
  3. 重庆医科大学重庆市高校市级口腔生物医学工程重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001034
  CLC： R739.86
- Published：2016，
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[1]王俊,季平,李立峰,高立娜,管超,苏可欣,李林林.顺铂调控舌鳞癌细胞增殖和迁移能力的研究[J].重庆医科大学学报,2016,41(10):1027-1032.

Wang Jun, Ji Ping, Li Lifeng, et al. Proliferation and migration ability of tongue squamous cells before and after exposure to cisplatin. [J]. Journal of Chongqing Medical University 41(10):1027-1032(2016)
[1]王俊,季平,李立峰,高立娜,管超,苏可欣,李林林.顺铂调控舌鳞癌细胞增殖和迁移能力的研究[J].重庆医科大学学报,2016,41(10):1027-1032. DOI： 10.13406/j.cnki.cyxb.001034.

Wang Jun, Ji Ping, Li Lifeng, et al. Proliferation and migration ability of tongue squamous cells before and after exposure to cisplatin. [J]. Journal of Chongqing Medical University 41(10):1027-1032(2016) DOI： 10.13406/j.cnki.cyxb.001034.

摘要

目的:探讨舌鳞癌细胞（human tongue squamous cell carcinoma cells

SCC）经顺铂化疗后的增殖和迁移能力的改变

及其对细胞增殖相关Nanog基因表达的调控作用。方法:本文应用5、4、3.5μg/m L顺铂分别作用于舌鳞癌细胞系SCC-15

处理时间分别为24、48、72 h（实验组）

未经顺铂处理的细胞为对照。流式细胞仪检测细胞凋亡率

q RT-PCR及Western blot检测2组细胞中Nanog的表达情况

划痕实验检测2组细胞迁移能力的变化。结果:经顺铂处理后细胞凋亡率增加

48 h后凋亡率显著增加（P=0.000）。q RT-PCR结果显示24、48、72 h组的Nanog基因相对表达量在顺铂处理的细胞中逐渐增加

48 h Nanog基因表达量与对照组相比

差异具有统计学意义（P=0.000）。Western blot实验结果进一步验证了顺铂处理的舌鳞癌细胞中Nanog蛋白相对表达量较对照组高（P≤0.001）。划痕实验显示经顺铂作用时间越长的实验组

细胞迁移速率越高。结论:顺铂化疗对舌鳞癌细胞增殖能力和迁移能力有调控作用。

Abstract

Objective:To determine the changes of proliferation and migration of human tongue squamous cell carcinoma cells(SCC)under cisplatin treatment and the key role of Nanog gene in those regulation. Methods:SCC-15 cell line was respectively treated by 5μg/m L cisplatin for 24 h

4 μg/m L for 48 h and 3.5 μg/m L for 72 h which were taken as experimental groups

while cells without cisplatin were blank control group. Flow cytometry was carried out to measure the cell apoptosis rate. q RT-PCR and Western blot were used to detect the expression of Nanog and wound healing test for measurement of cell migration ability. Results:Cell apoptosis rates were significantly increased with cisplatin treatment especially after 48 h. q RT-PCR and Western blot results confirmed the increased expression of Nanog at 24 h

48 h

72 h treatment point

which was significantly higher than that in control. Scratch assay also showed that the migration speed accelerate as the prolongation of time exposed to cisplatin. Conclusion:The proliferation and migration ability of SCC cells could be regulated by cisplatin.

关键词

舌鳞状细胞癌Nanog增殖迁移

Keywords

tongue squamous cell carcinomaNanogproliferationcell migration

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