Objective:To study the effects of Lingo-1 on neuron axon with Mg

2+

-free-induced seizure-like discharge. Methods:（1）Cortical neurons cultured for 10 d were exposed to normal media（normal group）or Mg

2+

-free media(Mg

2+

-free group)for 3 h and the morphology changes of neuron axon were observed with immunofluorescence.（2）The m RNA and protein expressions of Lingo-1 were detected by q RT-PCR and Western blot at 6

12

24

48 and 72 h after Mg

2+

-free or normal media treatment.（2）The expression of Lingo-1in neuron was inhibited by lentivirus-mediated RNA. Cortical neurons were divided into normal group

Mg

2+

-free group

Mg

2+

-free +control sh RNA group and Mg

2+

-free + Lingo-1sh RNA group. The morphology changes of neuron axon were observed and the neuron axon length was detected with immunofluorescence. Results :（1）In normal group

neurons axon was thick and in dense network connection. NF200 only expressed in the neurite. In Mg

2+

-free group

neurons axon was slender and in sparse network connection.NF200 highly expressed in cell body.（2）From 6 h to 72 h

Lingo-1 expression was increased at each point in Mg

2+

-free group than in normal group（P

<

0.05）.（3）Mg

2+

-free group axon length was shorter than that in normal group（P=0.000）. There was no statistical difference in axon length between Mg

2+

-free group and Mg2 +- free + control sh RNA group. Axon length was longer in Mg

2+

- free +lentivirus group than in Mg

2+

-free+control sh RNA group（P = 0. 000）. Conclusion :Lingo- 1 may be involved in axon injury after Mg

2+

-free-induced neuron seizure-like discharge.