Establishment of mice model of pure psychological stress by combined stimuli:an experimental study

Xu Dongbao; Wang Jie; Yu Yanni; Wang Yuanhe; Huang Jiang; Dai Jialin; Xia Bing

doi:10.13406/j.cnki.cyxb.001017

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Establishment of mice model of pure psychological stress by combined stimuli:an experimental study

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- Establishment of mice model of pure psychological stress by combined stimuli:an experimental study
- Journal of Chongqing Medical University Vol. 41, Issue 8, Pages: 832-835(2016)
- 作者机构：
  
  贵州医科大学法医学系
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001017
  CLC： R749;R-332
- Published：2016，
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[1]徐东宝,王杰,于燕妮,汪元河,黄江,戴佳琳,夏冰.联合应激法建立单纯心理应激动物实验模型的研究[J].重庆医科大学学报,2016,41(08):832-835.

Xu Dongbao, Wang Jie, Yu Yanni, et al. Establishment of mice model of pure psychological stress by combined stimuli:an experimental study. [J]. Journal of Chongqing Medical University 41(8):832-835(2016)
[1]徐东宝,王杰,于燕妮,汪元河,黄江,戴佳琳,夏冰.联合应激法建立单纯心理应激动物实验模型的研究[J].重庆医科大学学报,2016,41(08):832-835. DOI： 10.13406/j.cnki.cyxb.001017.

Xu Dongbao, Wang Jie, Yu Yanni, et al. Establishment of mice model of pure psychological stress by combined stimuli:an experimental study. [J]. Journal of Chongqing Medical University 41(8):832-835(2016) DOI： 10.13406/j.cnki.cyxb.001017.

摘要

目的:应用联合应激法建立单纯心理应激小鼠实验模型。方法:雄性昆明小鼠30只

随机分为2组

分别为心理应激（psychological stress

PS）组和正常对照（normal control

NC）组。适应环境1周后

连续给予PS组小鼠隔离应激30 d

并每天随机给予捕食应激或社会失败应激1次;NC组群居饲养。末次应激后第2天进行旷场实验

断颈取血

通过酶联免疫吸附试验（enzyme-linked immuno-sorbent assay

ELISA）检测血清中促肾上腺皮质激素释放激素（corticotropin releasing hormone

CRH）、促肾上腺皮质激素（adrenocorticotropic hormone

ACTH）、皮质醇（cortisol

CORT）水平

并与NC组进行比较。结果:PS组经联合心理应激后

5 min旷场活动明显减少（P<0.01）。PS组与NC组比较

血清中CRH、ACTH及CORT水平均明显升高

差异具有统计学意义（P<0.01）。结论:通过联合应激法

可建立一种可靠的单纯心理应激动物实验模型。

Abstract

Objective:To build the mice model of pure psychological stress based on combined stimuli. Methods:Thirty male mice were randomized into 2 groups:psychological stress group(n =15) and control group(n =15). After one-week acclimation

the mice model of psychological stress was subjected to social isolation stress(30 days)

and randomized to predator stress(1/day) or social defeat stress(1/day). Open-field test was performed at 24 h after the last psychological stress. Then

the average contents of corticotropin releasing hormone(CRH)

adrenocorticotropic hormone(ACTH)and cortisol(CORT) detected by ELISA and were compared between two groups. Results:The activity was significantly reduced in open-field test in psychological stress group(P<0.01). The average contents of CRH

ACTH and CORT were significantly higher in psychological stress group than in control group(P<0.01).Conclusion:It is a credible method to establish animal model of pure psychological stress through combined stimuli.

关键词

心理应激动物实验模型捕食应激社会失败应激隔离应激

Keywords

Psychological Stressexperimental animal modelpredator stresssocial defeat stresssocial isolation stress

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