Significance of LKB1 and TGF-β1 expression in human prostate cancer tissues and cell lines

Lü Yuyu; Deng Wei; Yang Mingqing; Liu Bin

doi:10.13406/j.cnki.cyxb.001008

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Significance of LKB1 and TGF-β1 expression in human prostate cancer tissues and cell lines

更新时间：2024-01-17

- Significance of LKB1 and TGF-β1 expression in human prostate cancer tissues and cell lines
- Journal of Chongqing Medical University Vol. 41, Issue 7, Pages: 761-766(2016)
- 作者机构：
  
  1. 重庆市第五人民医院病理科
  2. 重庆医科大学基础医学院病理教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001008
  CLC： R737.25
- Published：2016，
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[1]吕玉宇,邓玮,杨明清,刘斌.前列腺癌组织和细胞中LKB1、TGF-β1的表达及意义[J].重庆医科大学学报,2016,41(07):761-766.

Lü Yuyu, Deng Wei, Yang Mingqing, et al. Significance of LKB1 and TGF-β1 expression in human prostate cancer tissues and cell lines. [J]. Journal of Chongqing Medical University 41(7):761-766(2016)
[1]吕玉宇,邓玮,杨明清,刘斌.前列腺癌组织和细胞中LKB1、TGF-β1的表达及意义[J].重庆医科大学学报,2016,41(07):761-766. DOI： 10.13406/j.cnki.cyxb.001008.

Lü Yuyu, Deng Wei, Yang Mingqing, et al. Significance of LKB1 and TGF-β1 expression in human prostate cancer tissues and cell lines. [J]. Journal of Chongqing Medical University 41(7):761-766(2016) DOI： 10.13406/j.cnki.cyxb.001008.

摘要

目的:研究前列腺癌组织及细胞株中丝氨酸-苏氨酸激酶11（liver kinase B1/serine-threonine kinase 11

LKB1）、转化生长因子-β1（transforming growth factor-β1

TGF-β1）的表达情况

探讨LKB1表达在前列腺癌发生演进中的作用。方法:用免疫组化SP法、Western blot和RT-PCR检测LKB1及TGF-β1在前列腺增生和前列腺癌组织

以及2种细胞株（正常前列腺上皮RWPE-1

前列腺癌细胞株PC-3）中的蛋白和m RNA表达情况。结果:前列腺癌组织中LKB1、TGF-β1的阳性表达率分别为55%、70%

前列腺增生组织中LKB1、TGF-β1的阳性表达率分别为83.3%、40%

前列腺癌组织中LKB1的表达显著低于前列腺增生组织（P<0.05）

前列腺癌组织中TGF-β1的表达明显高于前列腺增生组织（P<0.05）

LKB1和TGF-β1在前列腺癌中的表达呈负相关（r=-0.373

P=0.018）;前列腺增生组织中LKB1的蛋白和m RNA表达水平明显高于前列腺癌组织（P<0.01）

前列腺癌组织中TGF-β1的蛋白和m RNA表达水平显著高于前列腺增生组织。免疫细胞化学、Western blot、RT-PCR结果显示:LKB1在人前列腺上皮细胞株RWPE-1中的蛋白及m RNA表达水平明显高于前列腺癌细胞株PC-3（P<0.01）;TGF-β1在前列腺癌细胞株PC-3中的蛋白及m RNA表达水平明显高于人前列腺上皮细胞株RWPE-1（P<0.01）。结论:LKB1在前列腺癌中表达下调

而TGF-β1在前列腺癌中过表达

LKB1在前列腺癌的发生过程中可能起着抑制TGF-β1的作用;对LKB1和TGF-β1相互作用机制的深入探讨有可能使其成为研究前列腺癌发生进展的分子机制新靶点。

Abstract

Objective:To observe the effect of liver kinase B1/serine-threonine kinase 11(LKB1)on the progression of prostate cancer by studying the expression of LKB1

transforming growth factor-β1(TGF-β1)in prostate cancer tissues and cells. Methods:Two cell lines(normal prostatic epithelial cell line RWPE-1

prostatic carcinoma cell lines PC-3)were detected by SP method

RT-PCR and Western blot in order to detect the expression of LKB

TGF-β1 m RNA and protein on human prostatic hyperplasia and prostate cancer tissues. Results :The positive rates of LKB1 and TGF- β1 were 55% and 70% in prostatic carcinoma

and 83.3% and 40%in prostatic hyperplasia. The positive rates of LKB1 were lower in prostatic carcinoma than in prostatic hyperplasia(P<0.05)

and the positive rates of TGF-β1were higher in prostatic carcinoma than in prostatic hyperplasia(P <0.05). However

LKB1 expression had a negative correlation with TGF-β1(r=-0.373

P=0.018). The relative levels of LKB1 protein and m RNA were higher in prostatic hyperplasia than in prostatic carcinoma(P<0.01). However

the relative levels of TGF-β1 protein and m RNA were higher in prostatic carcinoma than in prostatic hyperplasia. The level of LKB1 protein and m RNA were higher in prostatic epithelial cell line RWPE-1than in prostatic carcinoma cell line PC-3(P<0.01)by immunocytochemistry

Western blot and RT-PCR analysis. However

the expression of TGF-β1was higher in prostatic carcinoma cell line PC-3 than in prostatic epithelial cell line RWPE-1(P<0.05). Conclusion:The down-regulated expression of LKB1

while the excessive expression of TGF-β1 may play synergetic roles in the prostatic carcinogenensis. LKB1 gene may be a potential target for researching the molecular of prostatic cancer tumorgenesis and evolution by exploring the interactive mechanism of LKB1 and TGF-β1.

关键词

前列腺增生前列腺腺癌细胞株中丝氨酸-苏氨酸激酶11转化生长因子-β1

Keywords

prostatic carcinomaprostatic hyperplasialiver kinase B1/serine-threonine kinase 11transforming growth factor-β1

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