Expression and function of LAMA4 in the placentas and trophoblast

Wang pingzhen; Shan Nan; Wang Yiming; Qi Hongbo

doi:10.13406/j.cnki.cyxb.001007

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Expression and function of LAMA4 in the placentas and trophoblast

更新时间：2024-01-17

- Expression and function of LAMA4 in the placentas and trophoblast
- Journal of Chongqing Medical University Vol. 41, Issue 7, Pages: 694-698(2016)
- 作者机构：
  
  1. 重庆医科大学附属第一医院产科
  2. 重庆市肿瘤研究所肿瘤内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001007
  CLC： R714.244
- Published：2016，
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[1]王平珍,单楠,王懿铭,漆洪波.LAMA4在胎盘组织中的差异表达及其在滋养细胞中的功能[J].重庆医科大学学报,2016,41(07):694-698.

Wang pingzhen, Shan Nan, Wang Yiming, et al. Expression and function of LAMA4 in the placentas and trophoblast. [J]. Journal of Chongqing Medical University 41(7):694-698(2016)
[1]王平珍,单楠,王懿铭,漆洪波.LAMA4在胎盘组织中的差异表达及其在滋养细胞中的功能[J].重庆医科大学学报,2016,41(07):694-698. DOI： 10.13406/j.cnki.cyxb.001007.

Wang pingzhen, Shan Nan, Wang Yiming, et al. Expression and function of LAMA4 in the placentas and trophoblast. [J]. Journal of Chongqing Medical University 41(7):694-698(2016) DOI： 10.13406/j.cnki.cyxb.001007.

摘要

目的:探讨层粘连蛋白α4链（laminin alpha4

LAMA4）在胎盘组织中的差异表达及其对滋养细胞功能的影响

揭示其在子痫前期（preeclampsia

PE）发病机制中的作用。方法:收集22例PE和20例正常产妇的胎盘组织

应用免疫组化方法检测LAMA4蛋白在胎盘组织中的表达。采用RNA干扰技术

将滋养细胞分为正常培养对照组、缺氧/复氧（hypoxia/reoxygenation

H/R）培养组及干扰培养组

采用细胞免疫荧光和Western blot法检测LAMA4蛋白在各组细胞中的表达。采用Transwell小室模型检测各培养组细胞的侵袭功能。结果:（1）子痫前期胎盘组织中LAMA4蛋白的表达水平明显低于对照组（P<0.05）。（2）H/R及干扰处理后滋养细胞中LAMA4蛋白的表达水平明显降低（P<0.05）。（3）下调LAMA4的蛋白表达后滋养细胞的侵袭率明显降低（P<0.05）。结论:LAMA4蛋白表达的下调可抑制滋养细胞的侵袭功能

可能参与子痫前期的发病。

Abstract

Objective:To investigate the expression of the laminin alpha4(LAMA4)gene and its effect on invasion of HTR-8/SVneo cells and its role in the pathogenesis of preeclampsia. Methods:Twenty-two patients with severe pre-eclampsia and 20 normal pregnant women were investigated. The expression of LAMA4 was determined by immunohistochemistry. Using RNA interference technology

the trophoblast cells were divided into the normal control group

hypoxia/reoxygenation(H/R)oxygen culture group and LAMA4 interference group. Expression of lama4 in each group was detected by the immune fluorescence and Western blot. The invasive ability of the HTR-8/SVneo cells was tested by transwell. Results:(1)The expression of LAMA4 in severe pre-eclampsia group was dramatically lower than that of control group.(2)The expression of LAMA4 in HTR-8/SVneo cells was significantly decreased after H/R and si RNA treatments.(3)The invasive ability of HTR-8/SVneo cells was deduced after the deduction of LAMA4 expression. Conclusion:Damage of LAMA4 is probably involved in the mechanism of onset of preeclampsia by regulating invasion of trophoblast.

关键词

层粘连蛋白α4链侵袭子痫前期

Keywords

laminin alpha4invasionpreeclampsia

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