Membrane estrogen receptor expression in breast cancer and its effect on epidermal growth factor

Xue Huichao; Wang Lei; Zheng Weiwei; Li Zexin; Wang Guoqiang; Guo Changlei; Li Jian; Wang Ying

doi:10.13406/j.cnki.cyxb.001000

您当前的位置：

首页 >

文章列表页 >

Membrane estrogen receptor expression in breast cancer and its effect on epidermal growth factor

更新时间：2024-01-17

- Membrane estrogen receptor expression in breast cancer and its effect on epidermal growth factor
- Journal of Chongqing Medical University Vol. 41, Issue 7, Pages: 756-760(2016)
- 作者机构：
  
  新乡医学院第一附属医院普外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001000
  CLC： R737.9
- Published：2016，
扫描看全文
[1]薛会朝,王雷,郑维维,李泽信,王国戗,郭长磊,李健,王迎.膜雌激素受体在乳腺癌中的表达及对表皮生长因子水平的影响[J].重庆医科大学学报,2016,41(07):756-760.

Xue Huichao, Wang Lei, Zheng Weiwei, et al. Membrane estrogen receptor expression in breast cancer and its effect on epidermal growth factor. [J]. Journal of Chongqing Medical University 41(7):756-760(2016)
[1]薛会朝,王雷,郑维维,李泽信,王国戗,郭长磊,李健,王迎.膜雌激素受体在乳腺癌中的表达及对表皮生长因子水平的影响[J].重庆医科大学学报,2016,41(07):756-760. DOI： 10.13406/j.cnki.cyxb.001000.

Xue Huichao, Wang Lei, Zheng Weiwei, et al. Membrane estrogen receptor expression in breast cancer and its effect on epidermal growth factor. [J]. Journal of Chongqing Medical University 41(7):756-760(2016) DOI： 10.13406/j.cnki.cyxb.001000.

摘要

目的:探讨了乳腺癌细胞中膜雌激素受体（membraneestrogenreceptor

m ER）表达情况以及对表皮生长因子水平的影响。方法:采用q RT-PCR和Western blot分析乳腺癌细胞乳腺癌细胞系-7（michigan cancer foundation-7

MCF-7）、MDA-MB-231和MDA-MB-435中膜雌激素受体m ER m RNA和蛋白质的表达水平。并选取中等表达的细胞采用慢病毒介导的m ER sh RNA建立m ER Knowdown的稳定细胞系

并分析膜雌激素白蛋白-E2（membrane estrogen-2

m E2）和传统的雌激素（estrogen-2

E2）分别对乳腺癌细胞（ERα+和ERα-）的生长影响及其对EGFR和EGF表达水平的影响。结果:3种乳腺癌细胞系中m ER均有表达

其中MCF-7细胞表达水平最高

MDA-MB-231和MDA-MB-435细胞表达水平相当。采用慢病毒介导m ER sh RNA建立的MCF-7稳定细胞系（MCF-7/ERα-）其m ER m RNA和蛋白质水平较正常MCF-7均明显下调（P<0.05）。传统E2均能促进MCF-7和MCF-7/ERα-细胞的增殖

无统计学意义（P>0.05）

但m E2作用后

MCF-7/ERα-生长速度明显低于MCF-7细胞（P<0.05）。传统E2刺激MCF-7和MCF-7/ERα-细胞后

细胞EGF m RNA和蛋白质水平无差异（P>0.05）

而m E2刺激MCF-7和MCF-7/ERα-细胞后

MCF-7/ERα-细胞EGF m RNA和蛋白质水平较MCF-7细胞水平明显下降（P<0.05）。结论:本研究有望为乳腺癌的治疗提供更新的分子治疗靶点

更精确的临床内分泌治疗预测指标。

Abstract

Objective:To investigate the expression of estrogen receptor in breast cancer cell and its effect on epidermal growth factor.Methods:The membrane estrogen receptor(m ER)expression at m RNA and protein levels in human breast cancer cell line MCF-7

MDA-MB-231 and MDA-MB-435 were detected by real time fluorescent quantitative PCR and Western blot. Stable m ER knowdown cell lines were constructed by lentiviral mediated m ER sh RNA. The effect of membrane estrogen(m E2) and traditional estrogen on growth of(ERα+ and ERα-)as well as on expression of epithelial growth factor receptor(EGFR)and epidermal growth factor(EGF)were analyzed by MTT. Results:m ER was expressed in MCF-7

MDA-MB-231 and MDA-MB-435 cell lines

with MCF-7 cell having the highest expression. m ER m RNA and protein levels in knowdown stable cells by lentiviral mediated sh RNA were significantly lower than those of MCF-7 cells(P<0.05). Estrogen can promote proliferation of MCF-7 and MCF-7/ER- cell and there was not statistical significance among three lines(P>0.05). But after treatment by m E2

the proliferation of MCF-7/ER- was significantly lower than that of MCF-7 cells(P<0.05). After MCF-7 and MCF-7/ER alpha cells being stimulated by estrogen

there was no statistical difference in EGF m RNA and protein levels between MCF-7/ER- cells and MCF-7(P>0.05). But after being stimulated by m ER

EGF m RNA and protein levels was significantly decreased in MCF-7/ER- cells than in MCF-7(P<0.05). Conclusion:This study is expected to provide a molecular therapeutic targeting for the treatment of breast cancer.

关键词

雌激素受体乳腺癌表皮生长因子

Keywords

membrane estrogen receptorbreast cancerEpidermal growth factor

references

Views

112

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Pathomics signature based on machine learning can predict the response to neoadjuvant chemotherapy in breast cancer patients

Effects of circular RNA hsa_circ_0002185 on cell viability and invasion of breast cancer cells and its mechanism of action

Prediction and identification of H-2Kd restricted CTL epitope derived from breast cancer associated antigen ROR1

Comparison of lung dosimetry between supine and sternal horizon positions during X-ray irradiated radical mastectomy radiation therapy

Relative factors of catheter-related thrombosis in 172 breast cancer patients with implantable intravenous port

Related Author

Zhang Jieqiu

Wu Qi

Wang Jianmei

Yao Xiaopeng

Shaojie Qin

Mingliang Ning

Qingyuan Liu

Zhenning Tang

Related Institution

Department of Epidemiology and Health Statistics， School of Public Health，Southwest Medical University

Department of Pathology，The Affiliated Hospital of Southwest Medical University

Department of Medical Engineering Technology，School of Medical Information and Engineering，Southwest Medical University

Oncological Surgery Third Department, General Hospital of Ningxia Medical University

Department of Pathophysiology,College of Basic Medicine,Chongqing Medical University,Laboratory of Stem Cell and Tissue Engineering

⁰