Clinical and biological significance of MYH9 in human hepatocellular carcinoma and its role in cell migration and invasion

Yan Jun; Liu Yong

doi:10.13406/j.cnki.cyxb.000991

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Clinical and biological significance of MYH9 in human hepatocellular carcinoma and its role in cell migration and invasion

更新时间：2024-01-17

- Clinical and biological significance of MYH9 in human hepatocellular carcinoma and its role in cell migration and invasion
- Journal of Chongqing Medical University Vol. 41, Issue 6, Pages: 593-597(2016)
- 作者机构：
  
  重庆市中医院肝病科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000991
  CLC： R735.7
- Published：2016，
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[1]严君,刘勇.MYH9在肝细胞癌中的临床意义及生物学功能研究[J].重庆医科大学学报,2016,41(06):593-597.

Yan Jun, Liu Yong. Clinical and biological significance of MYH9 in human hepatocellular carcinoma and its role in cell migration and invasion. [J]. Journal of Chongqing Medical University 41(6):593-597(2016)
[1]严君,刘勇.MYH9在肝细胞癌中的临床意义及生物学功能研究[J].重庆医科大学学报,2016,41(06):593-597. DOI： 10.13406/j.cnki.cyxb.000991.

Yan Jun, Liu Yong. Clinical and biological significance of MYH9 in human hepatocellular carcinoma and its role in cell migration and invasion. [J]. Journal of Chongqing Medical University 41(6):593-597(2016) DOI： 10.13406/j.cnki.cyxb.000991.

摘要

目的:检测非肌性肌球蛋白重链9（non-muscle myosin heavy chain 9

MYH9）在人肝细胞癌（hepatocellular carcinoma

HCC）中的表达及对HCC细胞迁移侵袭能力的影响。方法:收集2013年1月1日至2014年1月1日间于我院肝胆外科行手术切除的65例HCC及对应癌旁组织

q RT-PCR检测MYH9的m RNA水平

免疫组织化学染色检测组织中MYH9的蛋白表达水平

分析MYH9蛋白表达与肿瘤临床特征间的相关性。采用si RNA敲低MHCC-97H细胞中MYH9的表达分别采用划痕愈合试验及Transwell侵袭小室检测沉默MYH9之后MHCC-97H细胞迁移及侵袭能力的变化。结果:MYH9在HCC组织中表达水平均明显高于对应癌旁组织;HCC组织中MYH9高表达与肿瘤数量（≥2个）及高TNM分期（Ⅲ+Ⅳ期）显著相关;si RNA可明显降低MHCC-97H细胞中MYH9的表达水平;下调MYH9表达能够明显抑制MHCC-97H细胞的迁移及侵袭能力。结论:MYH9在肝细胞癌组织中表达上调并与肿瘤恶性临床病理特征有关

下调MYH9能够通过抑制肝癌细胞的迁移及侵袭而实现抗肿瘤效应。

Abstract

Objective:To detect the expression of non-muscle myosin heavy chain 9(MYH9)in human hepatocellular carcinoma(HCC)and its role in cell invasion. Methods:q RT-PCR and immunohistochemistry was applied to detect the expression of MYH9 in HCC(n=65)and matched tumor-adjacent tissues. The correlation between MYH9 expression and clinical features was analyzed by Pearson chi-square test. si RNA was used to knock-down the expression of MYH9 in MHCC-97 H cells. The effects of MYH9 on cell migration and invasion were measured by wound healing assay and transwell assay

respectively. Results:Both the m RNA and protein expression of MYH9 were significantly up-regulated in HCC tissues

and the positive expression of MYH9 was associated with the number of nodules(≥2)and advanced TNM stage(Ⅲ+Ⅳ stage). Down-regulating the expression of MYH9 could inhibit migration and invasion in MHCC-97 H cells. Conclusion:The expression of MYH9 is significantly higher in HCC tissues. Down-regulation of MYH9 could achieve an anti-cancer role through inhibiting cell migration and invasion.

关键词

非肌性肌球蛋白重链9肝细胞癌临床意义迁移侵袭

Keywords

non-muscle myosin heavy chain 9hepatocellular carcinomaclinical significancemigrationinvasion

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