Effects of MEST/PEG1 on cell migration and invasion in the human trophoblast cells and its roles in preeclampsia

Peng Wei; Chen Ying; Shan Nan; Ding Yubin; Qi Hongbo

doi:10.13406/j.cnki.cyxb.000984

您当前的位置：

首页 >

文章列表页 >

Effects of MEST/PEG1 on cell migration and invasion in the human trophoblast cells and its roles in preeclampsia

更新时间：2024-01-17

- Effects of MEST/PEG1 on cell migration and invasion in the human trophoblast cells and its roles in preeclampsia
- Journal of Chongqing Medical University Vol. 41, Issue 7, Pages: 653-657(2016)
- 作者机构：
  
  1. 重庆医科大学附属第一医院妇产科重庆医科大学中国-加拿大-新西兰联合母胎医学实验室
  2. 重庆医科大学公共卫生管理学院生殖生物学实验室重庆医科大学中国-加拿大-新西兰联合母胎医学实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000984
  CLC： R714.244
- Published：2016，
扫描看全文
[1]彭威,陈莹,单楠,丁裕斌,漆洪波.MEST/PEG1对绒毛外滋养层细胞侵袭和迁移能力的影响及其在子痫前期中的意义[J].重庆医科大学学报,2016,41(07):653-657.

Peng Wei, Chen Ying, Shan Nan, et al. Effects of MEST/PEG1 on cell migration and invasion in the human trophoblast cells and its roles in preeclampsia. [J]. Journal of Chongqing Medical University 41(7):653-657(2016)
[1]彭威,陈莹,单楠,丁裕斌,漆洪波.MEST/PEG1对绒毛外滋养层细胞侵袭和迁移能力的影响及其在子痫前期中的意义[J].重庆医科大学学报,2016,41(07):653-657. DOI： 10.13406/j.cnki.cyxb.000984.

Peng Wei, Chen Ying, Shan Nan, et al. Effects of MEST/PEG1 on cell migration and invasion in the human trophoblast cells and its roles in preeclampsia. [J]. Journal of Chongqing Medical University 41(7):653-657(2016) DOI： 10.13406/j.cnki.cyxb.000984.

摘要

目的:通过研究人中胚层特异性转录基因（mesoderm-specific transcript

MEST）对人绒毛滋养细胞迁移和侵袭等生物学功能的影响

探讨其在子痫前期（preeclampsia

PE）中发病的机制。方法:用免疫组化法和Western blot法检测正常和PE患者胎盘中MEST及DNA甲基转移酶（DNA methyltransferase

DNMT）的表达。并检测2组胎盘中MEST甲基化水平。用Western blot法检测缺氧/复氧（hypoxia/reoxygenation

H/R）和MEST si RNA处理人滋养细胞株HTR8/Svneo后缺氧诱导因子（hypoxia-inducible factor 1α

HIF1α）及MEST表达水平;并检测si RNA转染后对各组细胞增殖、迁移和侵袭能力的影响。结果:MEST在正常胎盘中的表达（4.524±0.586）明显高于PE胎盘（2.640±0.334）且在PE胎盘中处于高甲基化状态。H/R后HIF1α表达明显升高（3.692±0.441

7.091±0.698）

而MEST表达明显降低（4.856±0.169

2.205±0.318）

特异性敲低MEST后HTR-8细胞侵袭和迁移能力均明显下降（1.057±0.061

0.551±0.029）（1.113±0.087

0.477±0.034）

差异均有统计学意义（P<0.05）;而增殖能力无明显影响（0.170±0.010

0.158±0.010）（P>0.05）。结论:推测MEST DNA甲基化异常可能影响滋养细胞的迁移和侵袭

从而参与子痫前期的发病。

Abstract

Objective:To evaluate the effects of mesoderm-specific transcript(MEST)on cell migration and invasion in the human trophoblast cells and its roles in preeclampsia. Methods:Immunohistochemistry and Western blot were used to determine the expression of MEST and DNA methyltransferases(DNMTs)in normal and preeclampsia(PE)placental tissues

and to detect the MEST promoter methylation level. HTR8/SVneo cells were treated by hypoxia/ reoxygenation(H/R)or MEST si RNA before detecting the MEST

hypoxia-inducible factor 1α(HIF1α)protein expression and the cell proliferation

migration and invasion ability

respectively. Results:MEST protein expressions were significantly decreased(4.524±0.586

2.640±0.334)and its gene promoters were hypermethylated in the PE placental tissues. After H/R treatment

the MEST expressions were downregulated(4.856±0.169

2.205±0.318)while HIF1αwere upregulated(3.692±0.441

7.091±0.698)(P<0.05). The cell proliferation was not affected by si RNA of MEST(0.170±0.010

0.158±0.010)(P >0.05)

while the migration and invasion rate of HTR-8/SVneo cells were significantly decreased(1.057 ±0.061

0.551±0.029)(1.113±0.087

0.477±0.034)(P<0.05). Conclusion:MEST/PEG1 DNA methylation represses trophoblast migration and invasion

a possible pathological mechanism of preeclampsia.

关键词

中胚层特异性转录基因/父源表达基因DNA甲基化滋养细胞侵袭子痫前期

Keywords

mesoderm-specific transcript/paternally expressed gene 1DNA methylationtrophoblast invasionpreeclampsia

references

Views

197

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Gadd45α on cell migration and invasion in the human trophoblast derived HTR8/SVneo cells

Application value of placental growth factor，soluble fms-like tyrosine kinase-1，and glycosylated fibronectin in the prediction of preeclampsia

Study on the diagnostic value of inflammation score system in preeclampsia

Value of abnormal methylation of cg08721802 locus in differential diagnosis of benign and malignant pulmonary nodules

Effect of GPR30 protein on function of trophoblast cells and its relationship with the development of preeclampsia

Related Author

Liu Xiru

Mu Huaqiao

Luo Xin

Qi Hongbo

Tang Ye

Yang Lan

Xiao Jianping

Shi Hao

Related Institution

Medical Department，Chongqing Health Centre for Women and Children

Department of Obstetrics and Gynaecology，the First Affiliated Hospital of Chongqing Medical University

Department of Medical Genetics and Prenatal Diagnosis，Wuxi Maternal and Child Health Hospital

Department of Obstetrics and Gynecology，Third Affiliated Hospital of Zunyi Medical University， Zunyi First People’s Hospital

Department of Clinical Laboratory，The Second Hospital of Shandong University/Tumor Marker Detection Engineering Laboratory of Shandong Province

⁰