Effects of curcumin on the expression of TNF-α,NF-κB,COX-2 in rats with autoimmune prostatitis

Zhao Liang; Chen Yuqin; Cheng Shaolong; Xiao Mingzhao

doi:10.13406/j.cnki.cyxb.000977

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Effects of curcumin on the expression of TNF-α,NF-κB,COX-2 in rats with autoimmune prostatitis

更新时间：2024-01-17

- Effects of curcumin on the expression of TNF-α,NF-κB,COX-2 in rats with autoimmune prostatitis
- Journal of Chongqing Medical University Vol. 41, Issue 9, Pages: 910-914(2016)
- 作者机构：
  
  重庆医科大学附属第一医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000977
  CLC： R285.5
- Published：2016，
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[1]赵亮,陈玉琴,程邵龙,肖明朝.姜黄素对免疫性前列腺炎大鼠肿瘤坏死因子-α、核因子-κB、环氧合酶-2表达的影响[J].重庆医科大学学报,2016,41(09):910-914.

Zhao Liang, Chen Yuqin, Cheng Shaolong, et al. Effects of curcumin on the expression of TNF-α,NF-κB,COX-2 in rats with autoimmune prostatitis. [J]. Journal of Chongqing Medical University 41(9):910-914(2016)
[1]赵亮,陈玉琴,程邵龙,肖明朝.姜黄素对免疫性前列腺炎大鼠肿瘤坏死因子-α、核因子-κB、环氧合酶-2表达的影响[J].重庆医科大学学报,2016,41(09):910-914. DOI： 10.13406/j.cnki.cyxb.000977.

Zhao Liang, Chen Yuqin, Cheng Shaolong, et al. Effects of curcumin on the expression of TNF-α,NF-κB,COX-2 in rats with autoimmune prostatitis. [J]. Journal of Chongqing Medical University 41(9):910-914(2016) DOI： 10.13406/j.cnki.cyxb.000977.

摘要

目的:探讨姜黄素对免疫性前列腺炎大鼠前列腺组织肿瘤坏死因子-α（tumor necrosis factor alpha

TNF-α）、核因子-κB（nuclear factorκB

NF-κB）、环氧合酶-2（cyclooxygenase-2

COX-2）表达的影响。方法:将60只SD雄性大鼠随机分为4组:正常对照组、模型组、姜黄素腹腔注射组及塞来昔布组（阳性对照组）

每组15只。大鼠腹腔注射百白破疫苗同时皮下多点注射经过乳化的前列腺蛋白提纯液和免疫佐剂构建免疫性前列腺炎模型。造模成功后

连续给药四周。酶联免疫吸附法（enzyme linked immunosorbent assay

ELISA）检测前列腺组织中TNF-α的表达

免疫组织化学法测定前列腺组织NF-κB（p65）水平变化

免疫荧光测定前列腺组织COX-2水平变化

Western blot法测定前列腺组织NF-κB（p65）及COX-2的表达。结果:模型组大鼠前列腺组织中TNF-α（F=382.730

P=0.000）、NF-κB（p65）（F=56.230

P=0.000）、COX-2（F=122.290

P=0.000）的表达明显高于其他3组。姜黄素治疗组大鼠前列腺组织中TNF-α、COX-2表达明显低于塞来昔布治疗组（P<0.01）

而NF-κB（p65）的表达无明显差异（P=0.059）。结论:姜黄素对免疫性前列腺炎大鼠有一定的治疗作用

其机制可能与降低前列腺组织中炎性细胞因子TNF-α、NF-κB（p65）、COX-2水平有关。

Abstract

Objective:To investigate the influence of curcumin on the expression of tumor necrosis factor-α(TNF-α)

nuclear factor κB(NF-κB)(p65)and cyclooxygenase-2(COX-2)in in rats with autoimmune prostatitis. Methods:Sixty male Sprague Dawley rats were equally and randomly divided into four groups:normal control group

model group

curcumin intraperitoneal injection group and celecoxib group(positive control group). The experimental autoimmune prostatitis rat model was made by intraperitoneal injection of vaccine and multiple hypodermic injection of prostate extract mixed with adjuvants. After the experimental animal model being estab-lished

each group was treated for four weeks. The levels of TNF-α in prostate tissues were detected by ELISA assay. The expressions of NF-κB(p65)and COX-2 in rat prostate tissues were respectively measured by immunohistochemistry

immunofluorescence and Western blot. Results:Levels of TNF-α(F=382.73

P=0.000)

NF-κB(p65)(F=56.23

P=0.000)and COX-2(F=122.29

P=0.000)in prostate tissues were significantly higher in model group than in the other groups. Expressions of TNF-a and COX-2 were obviously lower in curcumin intraperitoneal injection group than in positive control group(P<0.01);no significant difference in the expression of NF-κB(p65) was observed between the two groups(P =0.059). Conclusion:Curcumin is effective in treating rats with autoimmune prostatitis

and the mechanism may be associated with the reduction of the cytokines TNF-α

NF-κB(p65)and COX-2 in prostate tissues.

关键词

慢性前列腺炎/慢性骨盆疼痛综合征大鼠模型姜黄素

Keywords

chronic prostatitis/chronic pelvic pain syndromerat modelcurcumin

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