Inflammatory cytokine interleukin-4 may induce cognitive function impairment via autophagy in normal mice

Gu Min; Lü Yang; Zhao Nan; Tian Qi; Zhang Man; Yang Wenxiu; Xiao Qian; Yu Weihua

doi:10.13406/j.cnki.cyxb.000975

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Inflammatory cytokine interleukin-4 may induce cognitive function impairment via autophagy in normal mice

更新时间：2024-01-17

- Inflammatory cytokine interleukin-4 may induce cognitive function impairment via autophagy in normal mice
- Journal of Chongqing Medical University Vol. 41, Issue 8, Pages: 823-826(2016)
- 作者机构：
  
  1. 重庆医科大学附属第一医院老年病科神经与心理疾病组
  2. 重庆医科大学神经科学中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000975
  CLC： R364.5
- Published：2016，
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[1]辜敏,吕洋,赵楠,田琦,张漫,杨文秀,肖倩,余维华.炎症因子IL-4可能通过自噬导致正常鼠认知功能下降[J].重庆医科大学学报,2016,41(08):823-826.

Gu Min, Lü Yang, Zhao Nan, et al. Inflammatory cytokine interleukin-4 may induce cognitive function impairment via autophagy in normal mice. [J]. Journal of Chongqing Medical University 41(8):823-826(2016)
[1]辜敏,吕洋,赵楠,田琦,张漫,杨文秀,肖倩,余维华.炎症因子IL-4可能通过自噬导致正常鼠认知功能下降[J].重庆医科大学学报,2016,41(08):823-826. DOI： 10.13406/j.cnki.cyxb.000975.

Gu Min, Lü Yang, Zhao Nan, et al. Inflammatory cytokine interleukin-4 may induce cognitive function impairment via autophagy in normal mice. [J]. Journal of Chongqing Medical University 41(8):823-826(2016) DOI： 10.13406/j.cnki.cyxb.000975.

摘要

目的:研究腹腔注射白细胞介素-4（interleukin-4

IL-4）对C57BL/6小鼠学习记忆能力的影响。方法:将10只C57BL/6小鼠随机分成2组:IL-4干预组和对照组。在注射IL-4 1 d后进行水迷宫实验

包括5天定位航行和1天空间探索实验。采用Western blot和RT-PCR对自噬标记物微管相关蛋白1轻链3（microtubule associated protein 1 light chain 3

MAP1LC3

LC3）进行检测。结果:IL-4注射组小鼠学习记忆能力较对照组明显下降（穿越平台次数:4.750±0.500 vs.6.500±0.577;t=4.583

P=0.004）。免疫印迹显示

IL-4干预组小鼠脑组织中LC3较对照组明显增多（皮质:0.760±0.091 vs.0.482±0.085;t=-6.702

P=0.000;海马:0.772±0.068 vs.0.486±0.047;t=-10.267

P=0.000）。RT-PCR同样验证了这个趋势（皮质:1.422±0.002 vs.1.000±0.000;t=-311.903

P=0.000;海马:1.434±0.005 vs.1.000±0.000;t=-162.197

P=0.000）。结论:IL-4可能通过大量增加小鼠自噬作用

降低小鼠的学习记忆能力。

Abstract

Objective:to explore the effect of interleukin-4(IL-4)on the cognitive function in C57BL/6 mice. Methods:Ten C57BL/6mice were randomly divided into two groups:IL-4 intervention group(the mice intraperitoneally injected with IL-4 at the concentration of 100 ng/kg)and the control group. Morris water maze including five days of place navigation and one day of spatial probe was performed on the next day after intraperitoneal injection of IL-4. Microtubule associated protein 1 light chain 3(MAP1LC3

LC3)was detected by Western blot and reverse transcription-polymerase chain reaction(RT-PCR). Results:Compared with those of control group

the level of learning and memory of the mice in IL-4 intervention group was significantly decreased(times of crossing platform:4.750±0.500 vs. 6.500±0.577;t=4.583

P=0.004). Western blot showed that the expression of LC3 was increased in the mice in IL-4 intervention group than in control group(cortex:0.760±0.091 vs. 0.482±0.085;t=-6.702

P=0.000;hippocampus:0.772±0.068 vs.0.486±0.047;t=-10.267

P=0.000). RT-PCR also verified this trend(cortex:1.422±0.002 vs. 1.000±0.000;t=-311.903

P=0.000;hippocampus:1.434 ± 0.005 vs. 1.000±0.000;t=-162.197

P=0.000). Conclusion:IL-4 affects the function of autophagy

and the excessive autophagy may cause the damage to cognitive function in the mice.

关键词

白细胞介素-4自噬认知功能C57BL/6小鼠

Keywords

interleukin-4autophagycognitive functionC57BL/6 mice

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