Effects of Roux-en-Y gastric bypass on liver glycogen content and expression of Gsk-3β/Gs in obese rats

Mu Song; Liu jiayu; Guo Wei; Zhang Shuping; Wang Zhihong; Zhang Jun

doi:10.13406/j.cnki.cyxb.000960

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Effects of Roux-en-Y gastric bypass on liver glycogen content and expression of Gsk-3β/Gs in obese rats

更新时间：2024-01-17

- Effects of Roux-en-Y gastric bypass on liver glycogen content and expression of Gsk-3β/Gs in obese rats
- Journal of Chongqing Medical University Vol. 41, Issue 6, Pages: 569-575(2016)
- 作者机构：
  
  1. 重庆医科大学附属第一医院胃肠外科
  2. 重庆医科大学附属第一医院内分泌科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000960
  CLC： R656.6
- Published：2016，
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[1]母松,刘佳雨,郭伟,张书平,汪志红,张军.Roux-en-Y胃转流术对肥胖大鼠肝脏糖原含量及Gsk-3β/Gs的影响[J].重庆医科大学学报,2016,41(06):569-575.

Mu Song, Liu jiayu, Guo Wei, et al. Effects of Roux-en-Y gastric bypass on liver glycogen content and expression of Gsk-3β/Gs in obese rats. [J]. Journal of Chongqing Medical University 41(6):569-575(2016)
[1]母松,刘佳雨,郭伟,张书平,汪志红,张军.Roux-en-Y胃转流术对肥胖大鼠肝脏糖原含量及Gsk-3β/Gs的影响[J].重庆医科大学学报,2016,41(06):569-575. DOI： 10.13406/j.cnki.cyxb.000960.

Mu Song, Liu jiayu, Guo Wei, et al. Effects of Roux-en-Y gastric bypass on liver glycogen content and expression of Gsk-3β/Gs in obese rats. [J]. Journal of Chongqing Medical University 41(6):569-575(2016) DOI： 10.13406/j.cnki.cyxb.000960.

摘要

目的:探讨Roux-en-Y胃转流术（Roux-en-Y gastric bypass

RYGB）对肥胖大鼠肝脏糖原合成酶激酶-3β（glycogen synthase kinase-3β

Gsk-3β）及糖原合成酶（glycogen synthase

Gs）表达的影响。方法:SD大鼠70只

随机选取60只高脂饲料喂养建立肥胖大鼠模型

其余作为正常对照组（Con组

n=10）

肥胖造模成功大鼠共计26只

按干预方式不同将肥胖大鼠分为肥胖组（n=8）、RYGB手术组（Ob+RYGB组

n=10）和假手术组（Ob+Sham组

n=8）。检测各组大鼠空腹血糖和血清胰岛素水平

葡萄糖耐量实验（glucose insulin tolerance tests

GTT）、胰岛素耐量实验（insulin tolerance tests

ITT）测定各组糖耐量及胰岛素耐量情况

PAS染色检测肝脏组织糖原沉积情况

荧光定量PCR和Western blot检测肝组织Gsk-3β和Gs m RNA及蛋白表达情况。结果:排除死亡后每组成功建模量分别为9、8、7、7只

与正常对照组比较

Ob组、Ob+Sham组大鼠体质量、空腹血糖、血浆胰岛素、胰岛素抵抗指数、GTT、ITT曲线下面积明显升高（P<0.05）

肝脏组织糖原沉积明显减少

Gsk-3β表达明显上调（P<0.05）

而Gs表达明显下调（P<0.05）;与Ob组和Ob+Sham组比较

Ob+RYGB大鼠体质量、空腹血糖、血清胰岛素、胰岛素抵抗指数、GTT、ITT曲线下面积明显下降（P<0.05）

肝脏组织糖原沉积明显增加

Gsk-3β表达明显下调（P<0.05）

Gs表达明显上调（P<0.05）。结论:RYGB手术可改善肥胖大鼠肝脏糖原合成代谢紊乱

其机制可能与其上调肝脏Gsk-3β和下调Gs蛋白的表达有关。

Abstract

Objective:To investigate the effect of Roux-en-Y gastric bypass(RYGB)on glycogen synthase kinase-3β(Gsk-3β)and glycogen synthase(Gs)

and to explore the mechanism of improving hepatic glycogen synthesis in obese rats. Methods:Sixty SD rats randomly chosen from 70 rats were fed with high-fat diet(HFD)

and the left 10 rats which fed with standardized diet were used as normal control group(n=10). Twenty-six rats in the HFD group having body weight at least 20% more than those in normal control rats were subdivided into three groups:obese group(Ob group

n=8)

obese RYGB group(Ob+RYGB group

n=10)

obese sham operation group(Ob +sham group

n =8). Intraperitoneal glucose and insulin tolerance tests(GTT and ITT) were performed after surgery.Hepatic glycogen deposition was observed using periodic acid schiff(PAS)staining. The m RNA levels and protein levels of Gsk-3βand Gs were examined with quantitative RT-PCR and Western blot respectively. Fasting blood glucose(FBG) and plasma levels of insulin also were tested at 8 week after surgery. Results:The number of rats in each group after successful modeling was 9

7 and 7

respectively. Compared with those in control group

the level of body weight

FBG

plasma insulin

homeostasis model assessment of insulin resistance

the area under curve of GTT and ITT were significantly increased(P<0.05)

hepatic glycogen content was decreased obviously

the expression of Gsk-3β were significantly increased(P<0.05)

and the expression of Gs were significantly decreased(P<0.05)in Ob group and Ob+Sham group

which were dramatically reversed by RYGB treatment(P<0.05). Conclusion:RYGB can significantly promote hepatic glycogen synthesis in obese rats

which may be related to the inhibition of Gsk-3β and improvement of Gs expression.

关键词

肥胖Roux-en-Y胃转流术肝脏糖原合成糖原合成酶激酶-3β糖原合成酶

Keywords

obesityRoux-en-Y gastric bypasshepatic glycogen synthesisglycogen synthase kinase-3β(Gsk-3β)glycogen synthase(Gs)

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