Influence of dihydroartemisinin on cell migration and invasion in human prostate cancer cells and its probable mechanism

Zou Wenqin; Ding Xuan; Tang Li; Xu Ge; Luo Ziguo

doi:10.13406/j.cnki.cyxb.000957

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Influence of dihydroartemisinin on cell migration and invasion in human prostate cancer cells and its probable mechanism

更新时间：2024-01-17

- Influence of dihydroartemisinin on cell migration and invasion in human prostate cancer cells and its probable mechanism
- Journal of Chongqing Medical University Vol. 41, Issue 9, Pages: 904-909(2016)
- 作者机构：
  
  1. 重庆医科大学生命科学研究院
  2. 第三军医大学大坪医院病理科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000957
  CLC： R737.25
- Published：2016，
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[1]邹文琴,丁璇,唐溧,许舸,罗子国.双氢青蒿素对前列腺癌细胞迁移和侵袭能力影响及机制的研究[J].重庆医科大学学报,2016,41(09):904-909.

Zou Wenqin, Ding Xuan, Tang Li, et al. Influence of dihydroartemisinin on cell migration and invasion in human prostate cancer cells and its probable mechanism. [J]. Journal of Chongqing Medical University 41(9):904-909(2016)
[1]邹文琴,丁璇,唐溧,许舸,罗子国.双氢青蒿素对前列腺癌细胞迁移和侵袭能力影响及机制的研究[J].重庆医科大学学报,2016,41(09):904-909. DOI： 10.13406/j.cnki.cyxb.000957.

Zou Wenqin, Ding Xuan, Tang Li, et al. Influence of dihydroartemisinin on cell migration and invasion in human prostate cancer cells and its probable mechanism. [J]. Journal of Chongqing Medical University 41(9):904-909(2016) DOI： 10.13406/j.cnki.cyxb.000957.

摘要

目的:探讨双氢青蒿素（dihydroartemisinin

DHA）对人前列腺癌细胞迁移和侵袭能力的影响及可能机制。方法:人前列腺癌细胞株PC-3与DU145经不同浓度（0、25、50、100μmol/L）的DHA处理48 h

运用细胞划痕和细胞侵袭实验检测细胞的迁移和侵袭能力。采用荧光定量PCR和Weston blot检测波形蛋白（Vimentin）和上皮-钙粘连素（E-Cadherin

CDH1）基因和蛋白表达水平。结果:不同浓度DHA均能显著抑制PC-3和DU145迁移和侵袭能力（P<0.001）。不同浓度DHA均能明显下调Vimentin m RNA和蛋白的表达（P<0.05）

上调CDH1 m RNA和蛋白的表达（P<0.05）。结论:双氢青蒿素能显著抑制人前列腺癌细胞的迁移和侵袭能力

其可能机制是DHA干扰Vimentin表达及诱导E-Cadherin表达

且高浓度DHA可能存在其他途径抑制前列腺癌细胞的迁移和侵袭能力。

Abstract

Objective:To investigate the effects of dihydroartemisinin(DHA)on cell migration and invasion in human prostate cancer cells and its underlying molecular mechanisms. Methods:PC-3 and DU145 cells were treated with different concentrations(0

100 μmol/L)of DHA for 48 h. The migration and invasion abilities of PC-3 and DU145 cells were examined using the cell migration and invasion assays. Vimentin and E-cadherin(CDH1)m RNA and protein expression levels were determined by RT-q PCR and Western blot respectively. Results:DHA can significantly inhibit cell migration and invasion of PC-3 and DU145 cells(P<0.001). The expression levels of Vimentin m RNA and protein were significantly decreased(P<0.05)

while the expression levels of CDH1 m RNA and protein were increased(P<0.05). Conclusion:DHA significantly inhibits the migration and invasion abilities of PC-3 and DU145 cells in vitro. These mechanisms may relate with the reduced expression of vimentin and the induced expression of CDH1 by DHA.In addition to inhibitting EMT

high concentration of DHA may inhibit the migration and invasion abilities of PC-3 and DU145 cells by other ways.

关键词

双氢青蒿素细胞侵袭细胞迁移人前列腺癌波形蛋白上皮-钙粘连素

Keywords

dihydroartemisinincell invasioncell migrationprostate cancerVimentinE-cadherin

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