Animal model of arteriovenous fistula combined with chronic renal failure in New Zealand white rabbits

Li Xiangjie; Li Zhui; Zhang Mao; Zhao Yu; Ren Wei; Cheng Jun

doi:10.13406/j.cnki.cyxb.000955

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Animal model of arteriovenous fistula combined with chronic renal failure in New Zealand white rabbits

更新时间：2024-01-17

- Animal model of arteriovenous fistula combined with chronic renal failure in New Zealand white rabbits
- Journal of Chongqing Medical University Vol. 41, Issue 9, Pages: 928-932(2016)
- 作者机构：
  
  重庆医科大学附属第一医院血管外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000955
  CLC： R-332;R692.5
- Published：2016，
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[1]李相杰,李追,张矛,赵渝,任为,成军.新西兰兔慢性肾功能衰竭合并动静脉内瘘模型的建立[J].重庆医科大学学报,2016,41(09):928-932.

Li Xiangjie, Li Zhui, Zhang Mao, et al. Animal model of arteriovenous fistula combined with chronic renal failure in New Zealand white rabbits. [J]. Journal of Chongqing Medical University 41(9):928-932(2016)
[1]李相杰,李追,张矛,赵渝,任为,成军.新西兰兔慢性肾功能衰竭合并动静脉内瘘模型的建立[J].重庆医科大学学报,2016,41(09):928-932. DOI： 10.13406/j.cnki.cyxb.000955.

Li Xiangjie, Li Zhui, Zhang Mao, et al. Animal model of arteriovenous fistula combined with chronic renal failure in New Zealand white rabbits. [J]. Journal of Chongqing Medical University 41(9):928-932(2016) DOI： 10.13406/j.cnki.cyxb.000955.

摘要

目的:建立慢性肾功能衰竭（chronic renal failure

CRF）合并颈部动静脉内瘘（arteriovenous fistula

AVF）的新西兰兔模型

研究CRF对AVF静脉内膜增生（venous neointimal hyperplasia

VNH）的影响。方法:32只健康新西兰兔随机等分为肾衰组和健康组

每组16只。肾衰组以腺嘌呤诱导新西兰兔CRF后建立AVF模型

健康组不做CRF诱导建立AVF模型。术后第4周取瘘口静脉段行HE染色评估内膜厚度、面积

免疫组织化学检测增殖细胞核抗原（proliferating cell nuclear antigen

PCNA）的表达评估其增殖活性

取肾组织行HE染色观察病理学改变。结果:肾衰组血尿素氮、肌酐水平于第4周明显增加

第6周时达正常值3倍左右

光镜下可见肾小管萎缩消失、结晶物沉着、间质纤维化伴炎症细胞浸润;肾衰组AVF静脉平均内膜厚度、面积及PCNA表达量均明显高于健康组（P﹤0.05）。结论:腺嘌呤可诱导家兔CRF形成

CRF对VNH具有促进作用

加快AVF的功能障碍

因此在研究AVF早期功能障碍时需建立CRF的AVF模型

以保证研究的科学性。

Abstract

Objective:To establish a rabbit model of chronic renal failure(CRF)combined with arteriovenous fistula(AVF)

and to investigate the effect of CRF on venous neointimal hyperplasia(VNH)of AVF. Methods:Thirty-two healthy rabbits were randomly divided into renal failure group and health group(16 in each group). Rabbits in renal failure group were fed chow containing adenine.After induction of CRF

an AVF model in neck was established in renal failure group. Whereas

the same AVF model with no intervention was established in health group. Four weeks after operation

the pathological changes of fistula tissue

specifically the thickness and area of intimal in venous segment was observed by HE staining;the proliferation activity of the neointimal cells was evaluated by immunohistochemical staining of proliferating cell nuclear antigen(PCNA);the pathological changes of renal tissue was observed by HE staining. Results:Compared with those in health group

the levels of SCr and BUN in the renal failure group were increased significantly in the first four weeks

and were three times higher than baseline at the sixth weeks. HE staining of renal tissue in renal failure group showed amounts of crystals deposition within the tubules with partial atrophy

and the renal interstitium infiltrated with inflammatory cells and fibrosis. Compared with those of health group

the average thickness and area of VNH and PCNA-positive cells in venous segment were increased significantly in the renal failure group(P<0.05). Conclusion:The CRF rabbit model is successfully established by feeding adnine

which promotes the VNH and AVF dysfunction. Therefore

AVF combined with CRF model should be established for the early AVF dysfunction studies in the further.

关键词

动静脉内瘘慢性肾功能衰竭腺嘌呤动物模型

Keywords

arteriovenous fistulachronic renal failureadenineanimal model

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Related Institution

Department of Endocrinology，Chongqing Traditional Chinese Medicine Hospital

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Department of Dermatology,The First Affiliated Hospital of Chongqing Medical University

Department of Gynaecology and Obstetrics,University Town Hospital of Chongqing Medical University

重庆医科大学传染病寄生虫病研究所

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