Objective:To investigate the mode and mechanism of Mi RNA376a/c targeted regulation of transforming growth factor alpha(TGFA)in CD133+ U251 glioma cell proliferation. Methods:Lentivirus clones of Mi R376a/c target regulation of TGFA was built and were divided into four groups according to Mi R376a/c and TGFA binding sites. Enzyme activity of each group was detected. CD133+U251 cells were divided into four groups according to the transfection in Mi R376a/c and Mi R-SCR. Cell culture and Gimsa staining were used to detect and observe TGFA protein expression

cell growth curve

and cell proliferation. Information was collected and sta tistical analysis was conducted. Results :There were statistically significant differences in Mi R376 a / c expression between CD133 +group and CD133-group(P=0.000). There were statistically significant differences in plasmid luciferase activity expression between wt-TGFA group and mut-TGFA group(P=0.000). Mi R376a/c lentivirus was imported into CD133+ U251 cells and TGFA protein ex pression was down regulated in Mi R376a/c group than in Mi R-SCR group and blank control group

with statistical differences(P<0.001). Growth curve test showed that with the extension of time

cell growth speed was slower in Mi R376 a group than in control group

with statistical significant differences(P=0.000). Conclusion:Mi RNA376a/c target regulation of TGFA inhibits clone and proliferation of CD133+U251 glioma cells.