Objective:To investigate the influence of Peroxiredoxin 6(Prdx6)on cerebral ischemia/-reperfusion(I/R)injury and its mechanism. Methods :The middle cerebral artery occlusion(MCAO)model was used to induce SD rats’ cerebral I/R on the left side. Rats(n =16) were randomly divided into Sham group

MCAO group

Scramble group and Prdx6-si RNA group;the former two groups did not receive lateral ventricle injection;Sham group only had surgery incision and line plug was not inserted;MCAO group received surgery;the latter two groups were respectively injected with 8 μl out-of-order Prdx6-si RNA and Prdx6-si RNA in lateral ventricle before surgery. In every group

nerve function score

brain water content and cerebral infarct volume were measured;HE and methylene blue staining method were used to detect the rats’ brain cortex neurons after I/R injury. The expressions of Prdx6 and Caspase3 protein were detected by Western blot. SOD and MDA activities were assayed and TUNLE staining was used to detect the related mechanism of apoptosis and oxidation. Results:The neurological function score

lateral ischemic cerebral water content and cerebral infarction volume were significantly increased(P=0.000)in Prdx6- si RNA group than in MCAO group;HE and methylene blue staining showed further aggravate cerebral ischemic neuronal damage in Prdx6-si RNA group than in MCAO group(P=0.000);SOD activity was further reduced(P=0.000)and MDA content was increased(P=0.000)in Prdx6-si RNA group than in MCAO group.TUNLE staining positive cells rate(P=0.000)and Caspase3 protein expression levels(P<0.01)were further increased in Prdx6-si R NA group than in MCAO group. There was no significant statistical difference in the above indicators between Scramble and MCAO groups. Conclusion:Prdx6 has the protective effect on cerebral I/R injury

which relates with antioxidant and antiapoptotic mechanisms.