Influence of tripterygium wilfordii polyglycoside on coagulation function of rats with ulcerative colitis

Zhou Ling; Zhou Biying; Tang Wentai

doi:10.13406/j.cnki.cyxb.000921

您当前的位置：

首页 >

文章列表页 >

Influence of tripterygium wilfordii polyglycoside on coagulation function of rats with ulcerative colitis

更新时间：2024-01-17

- Influence of tripterygium wilfordii polyglycoside on coagulation function of rats with ulcerative colitis
- Journal of Chongqing Medical University Vol. 41, Issue 7, Pages: 751-755(2016)
- 作者机构：
  
  1. 遵义医学院附属医院中医肛肠科
  2. 遵义医学院寄生虫学教研室
  3. 遵义医学院附属医院病理科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000921
  CLC： R574.62
- Published：2016，
扫描看全文
[1]周泠,周必英,唐文台.雷公藤多甙对溃疡性结肠炎大鼠凝血功能的影响[J].重庆医科大学学报,2016,41(07):751-755.

Zhou Ling, Zhou Biying, Tang Wentai. Influence of tripterygium wilfordii polyglycoside on coagulation function of rats with ulcerative colitis. [J]. Journal of Chongqing Medical University 41(7):751-755(2016)
[1]周泠,周必英,唐文台.雷公藤多甙对溃疡性结肠炎大鼠凝血功能的影响[J].重庆医科大学学报,2016,41(07):751-755. DOI： 10.13406/j.cnki.cyxb.000921.

Zhou Ling, Zhou Biying, Tang Wentai. Influence of tripterygium wilfordii polyglycoside on coagulation function of rats with ulcerative colitis. [J]. Journal of Chongqing Medical University 41(7):751-755(2016) DOI： 10.13406/j.cnki.cyxb.000921.

摘要

目的:观察雷公藤多甙对溃疡性结肠炎（ulcerative colitis

UC）大鼠凝血功能的影响。方法:将64只雄性清洁级SD大鼠随机分为模型组52只和正常对照组12只。正常对照组不做任何处理

模型组采用2

4-二硝基氯苯和醋酸复合法制作UC大鼠模型。于造模第19天

随机处死正常对照鼠和模型鼠各2只

确定造模成功后

将造模大鼠随机分成5组:模型对照组、肝素治疗对照组、雷公藤多甙低剂量组、雷公藤多甙中剂量组、雷公藤多甙高剂量组

每组10只。正常对照组和模型对照组不给药物

肝素治疗对照组尾静脉注射肝素钠50 U/100 g

雷公藤多甙低、中、高剂量组分别灌胃37.5 mg/kg、75 mg/kg和150 mg/kg

连续给药15 d。各组大鼠腹主动脉取血

一部分枸橼酸钠抗凝

用全自动血凝分析仪检测大鼠血浆活化部分凝血活酶时间（activated partial thromboplastin time

APTT）、凝血酶原时间（prothrombin time

PT）、纤维蛋白原（fibrinogen

FIB）水平;另一部分（ethylene diamine tetraacetic acid

EDTA）抗凝

用全自动血球分析仪检测大鼠血小板（platelet

PLT）数量。同时剖杀大鼠取结肠

观察结肠组织形态学及病理学变化。结果:大鼠PT和APTT

雷公藤多甙低[（7.18±0.22）s

（17.89±0.26）s]、中[（7.44±0.16）s

（19.87±0.46）s]和高[（7.72±0.13）s

（22.87±1.19）s]剂量组均低于正常对照组[（8.75±0.16）s

（24.79±0.77）s]（P<0.05）

高于模型对照组[（6.51±0.27）s

（16.82±0.42）s]（P<0.05）。FIB和PLT

雷公藤多甙低[（2.39±0.12）g/L

（1050.20±28.42）×109/L]、中[（2.33±0.12）g/L

（969.60±15.36）×109/L]和高[（1.91±0.06）g/L

（907.60±14.59）×109/L]剂量组均高于正常对照组[（1.69±0.12）g/L

（840.80±26.67）×109/L]（P<0.05）

低于模型对照组[（3.12±0.44）g/L

（1224.80±75.06）×109/L]（P<0.05）。雷公藤多甙各剂量组结肠组织炎症较模型对照组明显减轻

结肠组织形态学评分和溃疡形成分级均明显降低。结论:雷公藤多甙能延长UC大鼠PT、APTT

降低FIB、PLT

从而减轻结肠炎症反应。

Abstract

Objective:To observe the influence of tripterygium wilfordii polyglycoside(TWP)on the coagulation function of rats with ulcerative colitis(UC). Methods:Sixty-four female clean grade Sprague-Dawley(SD)rats were randomly divided into 52 model rats and 12 normal control rats. Rats in normal control group were left intact. Rats in model group were used to establish UC rat model by a compound method of 2

4-dinitrochlorobenzene(DNCB) and acetic acid. On the 19 th day

two rats were killed in normal control group and model group

respectively. After the UC rat model being established successfully

UC model rats were randomly divided into5 groups with each group of 10 rats:model control group

heparin treatment control group

TWP low dose group

TWP moderate dose group

TWP high dose group. Rats in normal control group and model control group were not given any drugs. Heparin treatment control group was injected with heparin sodium through caudal vein. TWP low dose group

moderate dose group and high dose group were administrated with TWP 37.5 mg/kg

75 mg/kg and 150 mg/kg

respectively. Rats were given drugs for 15 days. The blood was collected from abdominal aorta;one part was anticoagulated by sodium citrate to detect activated partial thromboplastin time(APTT)

prothrombin time(PT)

fibrinogen(FIB)using automatic blood coagulation analyzer;another part was anticoagulated by ethylene diamine tetraacetic acid(EDTA)to detect platelet(PLT)using automatic blood cell analyzer. Moreover

all rats were killed to collect colon tissue. The morphological and pathological changesof colon tissue were observed. Results:For PT and APTT

TWP low dose group[(7.18±0.22)s

(17.89±0.26)s]

moderate dose group[(7.44 ±0.16) s

(19.87 ±0.46) s] and high dose group [(7.72 ±0.13) s

(22.87 ±1.19) s] were lower than normal control group[(8.75±0.16)s

(24.79±0.77)s](P<0.05)

and higher than model control group[(6.51 ± 0.27)s

(16.82±0.42)s](P<0.05). For FIB and PLT

TWP low dose group[(2.39±0.12)g/L

(1 050.20±28.42)×109/L]

moderate dose group[(2.33±0.12)g/L

(969.60±15.36)×109/L] and high dose group[(1.91±0.06)g/L

(907.60±14.59)×109/L] were higher than normal control group[(1.69±0.12)g/L

(840.80±26.67)×109/L](P<0.05)and lower than model control group[(3.12 ± 0.44)g/L

(1 224.80±75.06)×109/L](P<0.05).Compared with those of model control group

there were less inflammation and lower morphology scoring and ulcer formation grading of colon tissue in rats of drug dose groups. Conclusion:TWP can prolong PT and APTT

and decrease FIB and PLT

thus alleviate inflammation in colon tissues of rats infected with ulcerative colitis.

关键词

溃疡性结肠炎雷公藤多甙凝血功能

Keywords

ulcerative colitistripterygium wilfordii polyglycosidecoagulation function

references

Views

117

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Intervention effect of coix seed polysaccharides on an in vitro inflammation model of ulcerative colitis and its mechanism: a study based on the NLRP3/caspase-1 pathway

A DNBS-induced model of ulcerative colitis in rats

Detection of Fas on colonic apoptosis epithelium in ulcerative colitis by double staining immunohistochemistry

Comparison of two experimental ulcerative colitis models in rat

Distribution of transplanted bone mesenchymal stem cells in colon of ulcerative colitis rat

Related Author

Wu Hang

Li Ying

Zhang Zaoyu

Chen Lijuan

Li Yanlong

Yang Weijian

Li Baoyu

Gou Yuqin

Related Institution

Department of Community Health and Chronic Non-communicable Disease Prevention and Control， Gansu Provincial Center for Disease Control and Prevention

Spleen and Stomach Disease Diagnosis and Treatment Center，Gansu Provincial Hospital of Traditional Chinese Medicine

School of Integrated Traditional Chinese and Western Medicine，Gansu University of Chinese Medicine

Department of General Surgery,The Second College of Clinical Medicine,Chongqing Medical University

重庆医科大学第二临床学院普外科

⁰