Effect of PLD2 gene silencing on proliferation and migration of colon cancer cells

Du Kunli; Liu Maoxi; Zhang Shouru; Wu Xingye; Zeng Li; Fu Zhongxue

doi:10.13406/j.cnki.cyxb.000896

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Effect of PLD2 gene silencing on proliferation and migration of colon cancer cells

更新时间：2024-01-17

- Effect of PLD2 gene silencing on proliferation and migration of colon cancer cells
- Journal of Chongqing Medical University Vol. 41, Issue 3, Pages: 217-222(2016)
- 作者机构：
  
  1. 重庆医科大学附属第一医院胃肠外科
  2. 重庆医科大学附属第一医院中西医结合科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.000896
  CLC： R735.35
- Published：2016，
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[1]杜昆利,刘茂希,张寿儒,吴星烨,曾丽,傅仲学.沉默PLD2基因对结肠癌细胞增殖和迁移能力的影响[J].重庆医科大学学报,2016,41(03):217-222.

Du Kunli, Liu Maoxi, Zhang Shouru, et al. Effect of PLD2 gene silencing on proliferation and migration of colon cancer cells. [J]. Journal of Chongqing Medical University 41(3):217-222(2016)
[1]杜昆利,刘茂希,张寿儒,吴星烨,曾丽,傅仲学.沉默PLD2基因对结肠癌细胞增殖和迁移能力的影响[J].重庆医科大学学报,2016,41(03):217-222. DOI： 10.13406/j.cnki.cyxb.000896.

Du Kunli, Liu Maoxi, Zhang Shouru, et al. Effect of PLD2 gene silencing on proliferation and migration of colon cancer cells. [J]. Journal of Chongqing Medical University 41(3):217-222(2016) DOI： 10.13406/j.cnki.cyxb.000896.

摘要

目的:探讨沉默磷脂酶D2（phospholipases D2

PLD2）基因的表达对人结肠癌细胞增殖和迁移能力的影响及其潜在的分子机制。方法:采用载有靶向PLD2的小干扰RNA（small interfering RNA

si RNA）腺病毒转染SW480和SW620细胞;q RTPCR和Western blot法检测转染后结肠癌细胞PLD2的m RNA和蛋白质表达的变化;平板克隆形成实验和Transwell小室分别检测各组细胞的增殖和细胞迁移能力的变化;采用Western blot法检测转染前后细胞中周期蛋白D1（Cyclin D1）和基质金属蛋白酶-2（metalloproteinase-2

MMP-2）蛋白的表达。结果:转染PLD2 si RNA后结肠癌细胞PLD2 m RNA和蛋白的表达量明显下降（均P<0.01）

沉默PLD2表达后细胞的增殖和迁移能力明显受抑制

同时Cyclin D1和MMP-2蛋白的表达量也明显下调（均P=0.000）。结论:干扰PLD2表达引起的细胞增殖和迁移能力的下降可能与Cyclin D1和MMP-2表达下调密切相关

提示PLD2可能成为结肠癌治疗的新靶点之一。

Abstract

Objective:To investigate the effect of silencing the PLD2(phospholipases D2)gene expression on cell proliferation and migration of colon cancer cell lines and to explore the underline molecular mechanisms. Methods:The small interfering RNA(si RNA)targeted PLD2 was transfected into SW480 and SW620 cells by adenovirus. Expression levels of PLD2 m RNA and protein were measured by q RT-PCR and Western blot

respectively. Plate clone formation assay and transwell chamber were used to detect the proliferation and migration ability of colon cancer cells. Finally

the expressions of Cyclin D1 and metalloproteinase-2(MMP-2)protein were measured by Western blot in both cell lines. Results:PLD2 si RNA greatly inhibited the expression of PLD2 in colon cancer cells at both m RNA and protein levels(all P<0.01). Down-regulated the PLD2 expression significantly suppressed the proliferation and migration ability of SW480 and SW620 cells and also inhibited the expression of Cyclin D1 and MMP-2 proteins in both cell lines(all P=0.000). Conclusion:Down- regulated PLD2 gene expression can clearly inhibit the proliferation and migration of colon cancer cells

which may closely associate with the decreased expression of Cyclin D1 and MMP-2

indicating a way of gene therapy for colon cancer.

关键词

磷脂酶D2细胞增殖细胞迁移结肠癌小干扰RNA

Keywords

phospholipases D2(PLD2)cell proliferationcell migrationcolon cancersmall interfering RNA

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